Aspergillus infection monitored by multimodal imaging in a rat model
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
27060291
DOI
10.1002/pmic.201500487
Knihovny.cz E-zdroje
- Klíčová slova
- Animal model, Aspergillosis, Biomedicine, Fungal infection, Inductively coupled plasma, Mass spectrometry, Multimodal imaging,
- MeSH
- Aspergillus izolace a purifikace patogenita MeSH
- aspergilóza diagnóza diagnostické zobrazování mikrobiologie MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací metody MeSH
- multimodální zobrazování metody MeSH
- plíce diagnostické zobrazování mikrobiologie patologie MeSH
- pozitronová emisní tomografie metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Although myriads of experimental approaches have been published in the field of fungal infection diagnostics, interestingly, in 21st century there is no satisfactory early noninvasive tool for Aspergillus diagnostics with good sensitivity and specificity. In this work, we for the first time described the fungal burden in rat lungs by multimodal imaging approach. The Aspergillus infection was monitored by positron emission tomography and light microscopy employing modified Grocott's methenamine silver staining and eosin counterstaining. Laser ablation inductively coupled plasma mass spectrometry imaging has revealed a dramatic iron increase in fungi-affected areas, which can be presumably attributed to microbial siderophores. Quantitative elemental data were inferred from matrix-matched standards prepared from rat lungs. The iron, silver, and gold MS images collected with variable laser foci revealed that particularly silver or gold can be used as excellent elements useful for sensitively tracking the Aspergillus infection. The limit of detection was determined for both (107) Ag and (197) Au as 0.03 μg/g (5 μm laser focus). The selective incorporation of (107) Ag and (197) Au into fungal cell bodies and low background noise from both elements were confirmed by energy dispersive X-ray scattering utilizing the submicron lateral resolving power of scanning electron microscopy. The low limits of detection and quantitation of both gold and silver make ICP-MS imaging monitoring a viable alternative to standard optical evaluation used in current clinical settings.
Citace poskytuje Crossref.org
Non-invasive and invasive diagnoses of aspergillosis in a rat model by mass spectrometry
Siderophores for molecular imaging applications
