Metabolic pathways of benzimidazole anthelmintics in harebell (Campanula rotundifolia)
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
27208642
DOI
10.1016/j.chemosphere.2016.05.015
PII: S0045-6535(16)30644-0
Knihovny.cz E-resources
- Keywords
- Albendazole, Biotransformation, Drug metabolism, Flubendazole, Phytoremediation,
- MeSH
- Albendazole metabolism MeSH
- Anthelmintics chemistry metabolism MeSH
- Benzimidazoles chemistry metabolism MeSH
- Biotransformation MeSH
- Campanulaceae cytology metabolism MeSH
- Ecosystem MeSH
- Feces chemistry MeSH
- Fenbendazole metabolism MeSH
- Cells, Cultured MeSH
- Mebendazole analogs & derivatives metabolism MeSH
- Metabolic Networks and Pathways * MeSH
- Tandem Mass Spectrometry methods MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Albendazole MeSH
- Anthelmintics MeSH
- Benzimidazoles MeSH
- Fenbendazole MeSH
- flubendazole MeSH Browser
- Mebendazole MeSH
Benzimidazoles anthelmintics, which enter into environment primarily through excretion in the feces or urine of treated animals, can affect various organisms and disrupt ecosystem balance. The present study was designed to test the phytotoxicity and biotransformation of the three benzimidazole anthelmintics albendazole (ABZ), fenbendazole (FBZ) and flubendazole (FLU) in the harebell (Campanula rotundifolia). This meadow plant commonly grows in pastures and comes into contact with anthelmintics through the excrements of treated animals. Suspensions of harebell cells in culture medium were used as an in vitro model system. ABZ, FLU and FBZ were not found to be toxic for harebell cells, which were able to metabolize ABZ, FLU and FBZ via the formation of a wide scale of metabolites. Ultrahigh-performance liquid chromatography coupled with high mass accuracy tandem mass spectrometry (UHPLC-MS/MS) led to the identification of 24, 18 and 29 metabolites of ABZ, FLU and FBZ, respectively. Several novel metabolites were identified for the first time. Based on the obtained results, the schemes of the metabolic pathways of these anthelmintics were proposed. Most of these metabolites can be considered deactivation products, but a substantial portion of them may readily be decomposed to biologically active substances which could negatively affect ecosystems.
References provided by Crossref.org
Benzimidazoles and Plants: Uptake, Transformation and Effect
The Identification of Metabolites and Effects of Albendazole in Alfalfa (Medicago sativa)
