The Role of SATB2 as a Diagnostic Marker of Sinonasal Intestinal-type Adenocarcinoma
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- adenokarcinom diagnóza metabolismus patologie MeSH
- diferenciální diagnóza MeSH
- dřevo MeSH
- imunohistochemie MeSH
- keratin-20 metabolismus MeSH
- kolorektální nádory diagnóza metabolismus patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- metaplazie MeSH
- nádorové biomarkery metabolismus MeSH
- nádory nosu diagnóza metabolismus patologie MeSH
- nádory tračníku diagnóza metabolismus patologie MeSH
- nosní dutina patologie MeSH
- paranazální dutiny patologie MeSH
- prognóza MeSH
- senioři MeSH
- střeva patologie MeSH
- transkripční faktor CDX2 metabolismus MeSH
- transkripční faktory metabolismus MeSH
- vazebné proteiny DNA v oblastech připojení k matrix metabolismus MeSH
- vystavení vlivu životního prostředí škodlivé účinky MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CDX2 protein, human MeSH Prohlížeč
- keratin-20 MeSH
- KRT20 protein, human MeSH Prohlížeč
- nádorové biomarkery MeSH
- SATB2 protein, human MeSH Prohlížeč
- transkripční faktor CDX2 MeSH
- transkripční faktory MeSH
- vazebné proteiny DNA v oblastech připojení k matrix MeSH
BACKGROUND: Intestinal-type adenocarcinoma (ITAC) of the nasal cavity and paranasal sinuses is an uncommon tumor associated with exposure to wood and leather dust, nickel, and possibly smoking. ITAC shares phenotypical features with colorectal carcinoma. In contrast to most non-intestinal-type sinonasal adenocarcinomas, ITAC is an aggressive adenocarcinoma with poor clinical outcome; therefore, its reliable separation from non-ITAC is very important. AIM: The use of a combination of immunohistochemical markers of intestinal differentiation was tested in a cohort of sinonasal carcinomas of different types. The aim of this study was to explore a new intestinal marker, SATB2, in conjunction with CDX2 and CK20 in differential diagnosis of sinonasal adenocarcinomas. MATERIALS AND METHODS: Seven ITACs, 66 non-ITACs, and 1 case of extensive intestinal metaplasia (IM) of the nasal mucosa were included in the study and stained with SATB2, CK20, CDX2, and CK7 antibodies. Detection of mismatch repair proteins was performed in all cases of ITAC. All 7 sinonasal ITACs have been tested for KRAS, NRAS, and BRAF gene mutations. RESULTS: All ITACs showed positive expression for SATB2, whereas all non-ITAC cases were negative. The only 1 case of IM was found to be positive for SATB2, whereas the same case showed negative expression of CK20 and only focal immunostaining for CDX2. The genetic analysis showed that only 1 sinonasal ITAC (1/7) showed KRAS c.35G>C, p.(Gly12Ala) mutation, whereas BRAF and NRAS genes were wild type. Four ITACs revealed wild-type KRAS, NRAS, and BRAF, and 2 remaining cases were not analyzable. All ITACs showed preserved nuclear expression of mismatch repair proteins. CONCLUSIONS: SATB2 in combination with CDX2 and CK20 differentiates sinonasal ITAC from non-ITAC with increased diagnostic sensitivity and specificity and detects IM in the sinonasal tract more easily.
Biopticka Lab Plzen Czech Republic
Department of Pathology Charles University Prague Faculty of Medicine in Plzen Plzen Czech Republic
Department of Pathology University of Calgary Calgary AB Canada
Molecular Pathology Laboratory Bioptic Laboratory Ltd Plzen Czech Republic
Oncological Clinic Charles University Prague Faculty of Medicine in Plzen Plzen Czech Republic
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