Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation
Jazyk angličtina Země Česko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27283756
DOI
10.5507/bp.2016.032
Knihovny.cz E-zdroje
- Klíčová slova
- chromosomal aberrations, comet assay, crude coal tar, genotoxicity, ultraviolet radiation,
- MeSH
- chromozomální aberace účinky léků účinky záření MeSH
- chronická nemoc MeSH
- dehet uhelný škodlivé účinky MeSH
- dospělí MeSH
- dvouřetězcové zlomy DNA účinky léků účinky záření MeSH
- keratolytika škodlivé účinky MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfocyty * MeSH
- psoriáza terapie MeSH
- senioři MeSH
- terapie ultrafialovými paprsky škodlivé účinky MeSH
- ultrafialové záření škodlivé účinky MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dehet uhelný MeSH
- keratolytika MeSH
AIMS: Goeckerman therapy is based on combined exposure to UV radiation (UVA, UVB) and crude coal tar (PAHs). Some indicators suggest a genotoxic hazard, however, the level of genotoxic risk of the therapy has not yet been investigated sufficiently. This study aims to assesss the genotoxic risk. METHODS: The studied group consisted of patients with chronic stable plaque psoriasis treated by Goeckerman therapy (n = 29). Heparin-treated peripheral blood samples were collected one day before the first treatment and immediately after the last procedure. The lymphocytes were isolated from the blood. The level of genotoxicity was evaluated using an alkaline version of the Comet assay which detects DNA single strand breaks (DNA-SSBs), a neutral version of the Comet assay which detects DNA double strand breaks (DNA-DSBs), and using chromosomal aberrations. RESULTS: The level of DNA-SSBs increased insignificantly (median; Q1-Q3): 1.4 (0.4; 0.1-1.4) vs. 2.5 (0.6; 0.3-2.7) %tDNA (P = 0.11) and the level of DNA-DSBs increased significantly: 7.8 (6.5; 3.4-10.5) vs. 20.7 (19.3; 14.2-24.6) % DNA (P < 0.001). The total number of aberrated cells (P < 0.001) and structurally aberrated cells (P < 0.001) increased significantly. CONCLUSION: The elevated levels of the DNA-DSBs and the chromosomal aberrations in the peripheral lymphocytes indicated a genotoxic hazard. However, the elevated level of the chromosomal abnormalities was below the upper level of the reference range for healthy Czech adults. While, the genotoxic risk appears to be low, Goeckerman treatment represents a further contribution to the lifetime load of genotoxic factors.
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