Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation
Language English Country Czech Republic Media print-electronic
Document type Journal Article
PubMed
27283756
DOI
10.5507/bp.2016.032
Knihovny.cz E-resources
- Keywords
- chromosomal aberrations, comet assay, crude coal tar, genotoxicity, ultraviolet radiation,
- MeSH
- Chromosome Aberrations drug effects radiation effects MeSH
- Chronic Disease MeSH
- Coal Tar adverse effects MeSH
- Adult MeSH
- DNA Breaks, Double-Stranded drug effects radiation effects MeSH
- Keratolytic Agents adverse effects MeSH
- Middle Aged MeSH
- Humans MeSH
- Lymphocytes * MeSH
- Psoriasis therapy MeSH
- Aged MeSH
- Ultraviolet Therapy adverse effects MeSH
- Ultraviolet Rays adverse effects MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Coal Tar MeSH
- Keratolytic Agents MeSH
AIMS: Goeckerman therapy is based on combined exposure to UV radiation (UVA, UVB) and crude coal tar (PAHs). Some indicators suggest a genotoxic hazard, however, the level of genotoxic risk of the therapy has not yet been investigated sufficiently. This study aims to assesss the genotoxic risk. METHODS: The studied group consisted of patients with chronic stable plaque psoriasis treated by Goeckerman therapy (n = 29). Heparin-treated peripheral blood samples were collected one day before the first treatment and immediately after the last procedure. The lymphocytes were isolated from the blood. The level of genotoxicity was evaluated using an alkaline version of the Comet assay which detects DNA single strand breaks (DNA-SSBs), a neutral version of the Comet assay which detects DNA double strand breaks (DNA-DSBs), and using chromosomal aberrations. RESULTS: The level of DNA-SSBs increased insignificantly (median; Q1-Q3): 1.4 (0.4; 0.1-1.4) vs. 2.5 (0.6; 0.3-2.7) %tDNA (P = 0.11) and the level of DNA-DSBs increased significantly: 7.8 (6.5; 3.4-10.5) vs. 20.7 (19.3; 14.2-24.6) % DNA (P < 0.001). The total number of aberrated cells (P < 0.001) and structurally aberrated cells (P < 0.001) increased significantly. CONCLUSION: The elevated levels of the DNA-DSBs and the chromosomal aberrations in the peripheral lymphocytes indicated a genotoxic hazard. However, the elevated level of the chromosomal abnormalities was below the upper level of the reference range for healthy Czech adults. While, the genotoxic risk appears to be low, Goeckerman treatment represents a further contribution to the lifetime load of genotoxic factors.
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