Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články
PubMed
27299774
DOI
10.1007/s00216-016-9664-z
PII: 10.1007/s00216-016-9664-z
Knihovny.cz E-zdroje
- Klíčová slova
- Blood sample, Nanoparticles, Pregnancy associated plasma protein A2, Surface plasmon resonance,
- MeSH
- kovové nanočástice chemie MeSH
- lidé MeSH
- limita detekce MeSH
- povrchová plasmonová rezonance metody MeSH
- pufry MeSH
- těhotenský plazmatický protein A analýza MeSH
- těhotenství MeSH
- zlato chemie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Názvy látek
- PAPPA2 protein, human MeSH Prohlížeč
- pufry MeSH
- těhotenský plazmatický protein A MeSH
- zlato MeSH
Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men. Graphical Abstract Temporal sensor response corresponding to respective steps of the assay for detection of PAPP-A2 in buffer.
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