Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27322763
DOI
10.1111/nph.14049
Knihovny.cz E-zdroje
- Klíčová slova
- AUX1, Arabidopsis, PIN-FORMED (PIN), auxin, cytokinins, root gravitropism,
- MeSH
- Arabidopsis účinky léků fyziologie MeSH
- biologické modely MeSH
- biologický transport účinky léků MeSH
- cytokininy farmakologie MeSH
- gravitace MeSH
- gravitropismus účinky léků MeSH
- kořeny rostlin účinky léků fyziologie MeSH
- kyseliny indoloctové metabolismus MeSH
- meristém účinky léků fyziologie MeSH
- proteiny huseníčku metabolismus MeSH
- signální transdukce účinky léků MeSH
- zelené fluorescenční proteiny metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- AUX1 protein, Arabidopsis MeSH Prohlížeč
- cytokininy MeSH
- kyseliny indoloctové MeSH
- proteiny huseníčku MeSH
- zelené fluorescenční proteiny MeSH
Redirection of intercellular auxin fluxes via relocalization of the PIN-FORMED 3 (PIN3) and PIN7 auxin efflux carriers has been suggested to be necessary for the root gravitropic response. Cytokinins have also been proposed to play a role in controlling root gravitropism, but conclusive evidence is lacking. We present a detailed study of the dynamics of root bending early after gravistimulation, which revealed a delayed gravitropic response in transgenic lines with depleted endogenous cytokinins (Pro35S:AtCKX) and cytokinin signaling mutants. Pro35S:AtCKX lines, as well as a cytokinin receptor mutant ahk3, showed aberrations in the auxin response distribution in columella cells consistent with defects in the auxin transport machinery. Using in vivo real-time imaging of PIN3-GFP and PIN7-GFP in AtCKX3 overexpression and ahk3 backgrounds, we observed wild-type-like relocalization of PIN proteins in the columella early after gravistimulation, with gravity-induced relocalization of PIN7 faster than that of PIN3. Nonetheless, the cellular distribution of PIN3 and PIN7 and expression of PIN7 and the auxin influx carrier AUX1 was affected in AtCKX overexpression lines. Based on the retained cytokinin sensitivity in pin3 pin4 pin7 mutant, we propose the AUX1-mediated auxin transport rather than columella-located PIN proteins as a target of endogenous cytokinins in the control of root gravitropism.
CEITEC Central European Institute of Technology Masaryk University Brno CZ 62500 Czech Republic
Institute of Science and Technology Klosterneuburg AT 3400 Austria
Citace poskytuje Crossref.org
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