Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
27417742
DOI
10.1016/j.anaerobe.2016.07.002
PII: S1075-9964(16)30086-5
Knihovny.cz E-resources
- Keywords
- Bacteria, Creole goats, NGS, PCR-DGGE, Rumen, qPCR,
- MeSH
- Actinobacteria classification genetics metabolism MeSH
- Rumen microbiology MeSH
- Bacteroidetes classification genetics metabolism MeSH
- Butyrivibrio classification genetics metabolism MeSH
- Clostridium classification genetics metabolism MeSH
- Fermentation MeSH
- Firmicutes classification genetics metabolism MeSH
- Phylogeny MeSH
- Animal Nutritional Physiological Phenomena * MeSH
- Hydrogen-Ion Concentration MeSH
- Goats MeSH
- Animal Feed analysis MeSH
- Zea mays chemistry metabolism MeSH
- Poaceae chemistry metabolism MeSH
- Gastric Fistula MeSH
- Prevotella classification genetics metabolism MeSH
- Ruminococcus classification genetics metabolism MeSH
- Sequence Analysis, DNA MeSH
- Gastrointestinal Microbiome physiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats to the high-grain diet (grain: hay - ratio of 40:60). The dietary transition of goats from the forage to the high-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higher than value established for acute or subacute ruminal acidosis was not diagnosed in studied animals. DGGE analysis demonstrated distinct ruminal microbial populations in hay-fed and grain-fed animals, but the substantial animal-to-animal variation were detected. Quantitative PCR showed for grain-fed animals significantly higher number of bacteria belonging to Clostridium leptum group at 10 days after the incorporation of corn into the diet and significantly lower concentration of bacteria belonging to Actinobacteria phylum at the day 20 after dietary change. Taxonomic distribution analysed by NGS at day 20 revealed the similar prevalence of the phyla Firmicutes and Bacteroidetes in all goats, significantly higher presence of the unclassified genus of groups of Bacteroidales and Ruminococcaceae in grain-fed animals and significantly higher presence the genus Prevotella and Butyrivibrio in the forage-fed animals. The three different culture-independent methods used in this study show that high proportion of concentrate in goat diet does not induce any serious disturbance of their rumen ecosystem and indicate the good adaptive response of caprine ruminal bacteria to incorporation of corn into the diet.
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