Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Chromosomal allocation, Chromosome genomics, Chromosome sorting, FISH, Flow cytometry, Single chromosome amplification, Transgene, Wheat,
- MeSH
- chromozomy rostlin * MeSH
- hybridizace in situ fluorescenční metody MeSH
- polymerázová řetězová reakce metody MeSH
- průtoková cytometrie metody MeSH
- pšenice genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Flow cytometry enables chromosomes to be sorted into different groups based on their characteristics, such as relative DNA content and the presence of repetitive DNA sequences. Despite the recent progress in the analysis of plant genome organization and chromosome structure, there is a need for easy methods to assign DNA sequences to individual chromosomes. Here, we describe an easy way to allocate genes or DNA sequences to chromosomes in wheat using flow-sorted chromosomes combined with fluorescence in situ hybridization and PCR analyses.
Citace poskytuje Crossref.org
