Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Chromosomal allocation, Chromosome genomics, Chromosome sorting, FISH, Flow cytometry, Single chromosome amplification, Transgene, Wheat,
- MeSH
- Chromosomes, Plant * MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Polymerase Chain Reaction methods MeSH
- Flow Cytometry methods MeSH
- Triticum genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Flow cytometry enables chromosomes to be sorted into different groups based on their characteristics, such as relative DNA content and the presence of repetitive DNA sequences. Despite the recent progress in the analysis of plant genome organization and chromosome structure, there is a need for easy methods to assign DNA sequences to individual chromosomes. Here, we describe an easy way to allocate genes or DNA sequences to chromosomes in wheat using flow-sorted chromosomes combined with fluorescence in situ hybridization and PCR analyses.
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