The study aims were focused on profiling eight hydrolytic enzymes by fluorescence method using a multifunctional modular reader and studying the proportion of basic microorganism groups during composting and vermicomposting of sewage sludge mixed with straw pellets in several proportions (0, 25, 50, 75, and 100%). The greatest decrease in enzymatic activity occurred in the first half of composting and vermicomposting. After 4 months of these processes, the least enzymatic activity was observed in the sludge with 50% and also 25% straw addition, indicating that straw is an important means for the rapid production of mature compost from sewage sludge. Enzymatic activity was usually less in the presence of earthworms than in the control treatment because some processes took place in the digestive tract of the earthworm. For the same reason, we observed reduced enzyme activity during fresh feedstock vermicomposting than precomposted material. The final vermicompost from fresh feedstocks exhibited less microbial biomass, and few fungi and G- bacteria compared to precomposted feedstock. The enzymatic activity during composting and vermicomposting of sewage sludge and their mixtures stabilized at the following values: β-D-glucosidase-50 μmol MUFG/h/g dw, acid phosphatase-200 μmol MUFP/h/g dw, arylsulphatase-10 μmol MUFS/h/g dw, lipase-1,000 μmol MUFY/h/g dw, chitinase-50 μmol MUFN/h/g dw, cellobiohydrolase-20 μmol MUFC/h/g dw, alanine aminopeptidase-50 μmol AMCA/h/g dw, and leucine aminopeptidase-50 μmol AMCL/h/g dw. At these and lesser values, these final products can be considered mature and stable.

Department of Agroenvironmental Chemistry and Plant Nutrition Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences Prague Czechia

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Albrecht R., Petit J. L., Calvert V., Terrom G., Périssol C. (2010). Changes in the level of alkaline and acid phosphatase activities during green wastes and sewage sludge co-composting. Bioresour. Technol. 101 228–233. 10.1016/j.biortech.2009.08.017 PubMed DOI

Almeida R. F., Naves E. R., Mota R. P. (2015). Soil quality: enzymatic activity of soil β-glucosidase. Glob. J. Agric. Res. Rev. 3 146–150.

Baldrian P. (2009). Microbial enzyme-catalyzed processes in soils and their analysis. Plant Soil Environ. 55 370–378. 10.17221/134/2009-pse DOI

Błońska E., Lasota J., Gruba P. (2017). Enzymatic activity and stabilization of organic matter in soil with different detritus inputs. J. Soil Sci. Plant Nutr. 63 242–247. 10.1080/00380768.2017.1326281 DOI

Bradshaw R. A. (2013). “Aminopeptidases,” in Encyclopedia of Biological Chemistry II, eds Lennarz W. J., Lane M. D. (Cambridge, MA: Academic Press; ), 97–99.

Domínguez J., Aira M., Crandall K. A., Pérez-Losada M. (2021). Earthworms drastically change fungal and bacterial communities during vermicomposting of sewage sludge. Sci. Rep. 11:15556. 10.1038/s41598-021-95099-z PubMed DOI PMC

Dominguez J., Edwards C. A. N. (2011). “Relationship between composting and vermicomposting,” in Vermiculture Technology: Earthworms, Organic Wastes, and Environmental Management, eds Edwards C. A., Arancon N. Q., Sherman R. (Boca Raton, FL: CRC Press; ), 11–25.

Domínguez J., Gómez-Brandón M. (2012). “Vermicomposting: composting with earthworms to recycle organic wastes,” in Management of Organic Waste, eds Kumar S., Sherman R. L. (London: IntechOpen; ), 29–48.

Estrella-González M. J., Jurado M. M., Suárez-Estrella F., López M. J., López-González J. A., Siles-Castellano A., et al. (2019). Enzymatic profiles associated with the evolution of the lignocellulosic fraction during industrial-scale composting of anthropogenic waste: comparative analysis. J. Environ. Manag. 248:109312. 10.1016/j.jenvman.2019.109312 PubMed DOI

Eurostat (2021). Available online at: http://appsso.eurostat.ec.europa.eu/nui/submitViewTableAction.do (accessed October 5, 2021).

García-Gil J. C., Plaza C., Soler-Rovira P., Polo A. (2000). Long-term effects of municipal solid waste compost application on soil enzyme activities and microbial biomass. Soil Biol. Biochem. 32 1907–1913.

García-Sánchez M., Stejskalová T., García-Romera I., Száková J., Tlustoš P. (2017). Risk element immobilization/stabilization potential of fungal-transformed dry olive residue and arbuscular mycorrhizal fungi application in contaminated soils. J. Environ. Manag. 201 110–119. 10.1016/j.jenvman.2017.06.036 PubMed DOI

Gea T., Ferrer P., Alvaro G., Valero F., Artola A., Sánchez A. (2007). Co-composting of sewage sludge:fats mixtures and characteristics of the lipases involved. Biochem. Eng. J. 33 275–283. 10.1016/j.bej.2006.11.007 DOI

Gómez-Brandón M., Aira M., Lores M., Domínguez J. (2011). Epigeic earthworms exert a bottleneck effect on microbial communities through gut associated processes. PLoS One 6:e24786. 10.1371/journal.pone.0024786 PubMed DOI PMC

Gunjal A. B., Waghmode M. S., Patil N. N., Nawani N. N. (2019). “Significance of soil enzymes in agriculture,” in Smart Bioremediation Technologies, Microbial Enzymes, ed. Bhatt P. (Cambridge, MA: Academic Press; ), 159–168.

Hanc A., Catkova T., Kuzel S., Cajthaml T. (2017). Dynamics of a vertical-flow windrow vermicomposting system. Waste Manag. Res. 11 1121–1128. 10.1177/0734242X17725161 PubMed DOI

Hanc A., Hrebeckova T., Grasserova A., Cajthaml T. (2021). Conversion of spent coffee grounds into vermicompost. Bioresour. Technol. 341:125925. 10.1016/j.biortech.2021.125925 PubMed DOI

Hemati A., Aliasgharzad N., Khakvar R., Khoshmanzar E., Lajayer B. A., van Hullebusch E. D. (2021). Role of lignin and thermophilic lignocellulolytic bacteria in the evolution of humification indices and enzymatic activities during compost production. Waste Manag. 119 122–134. 10.1016/j.wasman.2020.09.042 PubMed DOI

Hřebečková T., Wiesnerová L., Hanč A. (2019). Changes of enzymatic activity during a large-scale vermicomposting process with continuous feeding. J. Clean. Prod. 239:118127. 10.1016/j.jclepro.2019.118127 DOI

Krsek M., Wellington E. M. H. (2001). Assessment of chitin decomposer diversity within an upland grassland. Anton. Leeuw. 79 261–267. 10.1023/a:1012043401168 PubMed DOI

Ma C., Hu B., Wei M., Zhao J., Zhang H. (2019). Influence of matured compost inoculation on sewage sludge composting: enzyme activity, bacterial and fungal community succession. Bioresour. Technol. 294:122165. 10.1016/j.biortech.2019.122165 PubMed DOI

Manga M., Camargo-Valero M. A., Anthonj C., Evans B. E. (2021). Fate of faecal pathogen indicators during faecal sludge composting with different bulking agents in tropical climate. Int. J. Hyg. Environ. Health 232:113670. 10.1016/j.ijheh.2020.113670 PubMed DOI

Mondini C., Fornasier F., Sinicco T. (2004). Enzymatic activity as a parameter for the characterization of the composting process. Soil Biol. Biochem. 36 1587–1594. 10.1016/j.soilbio.2004.07.008 DOI

Nabarlatz D., Stüber F., Font J., Fortuny A., Fabregat A., Bengoa C. (2012). Extraction and purification of hydrolytic enzymes from activated sludge. Resour. Conserv. Recyc. 59 9–13. 10.1016/j.resconrec.2011.06.017 DOI

Nomenclature Committee of the International Union of Biochemistry and Molecular Biology [NC-IUBMB] (2021). Available online at: https://iubmb.qmul.ac.uk/enzyme/ (accessed October 1, 2021).

Poulsen P. H. B., Møller J., Magid J. (2008). Determination of a relationship between chitinase activity and microbial diversity in chitin amended compost. Bioresour. Technol. 99 4355–4359. 10.1016/j.biortech.2007.08.042 PubMed DOI

Robledo-Mahón T., Gómez-Silván C., Andersen G. L., Calvo C., Aranda E. (2020). Assessment of bacterial and fungal communities in a full-scale thermophilic sewage sludge composting pile under a semipermeable cover. Bioresour. Technol. 298:122550. 10.1016/j.biortech.2019.122550 PubMed DOI

Sethupathy A., Arun C., Sivashanmugam P., Ranjith Kumar R. (2020). Enrichment of biomethane production from paper industry biosolid using ozonation combined with hydrolytic enzymes. Fuel 279:118522. 10.1016/j.fuel.2020.118522 DOI

Štursová M., Baldrian P. (2011). Effects of soil properties and management on the activity of soil organic matter transforming enzymes and the quantification of soil-bound and free activity. Plant Soil 338 99–110. 10.1007/s11104-010-0296-3 DOI

Tejada M., Garcia C., Gonzalez J. L., Hernandez M. T. (2006). Use of organic amendment as a strategy for saline soil remediation: influence on the physical, chemical and biological properties of soil. Soil Biol. Biochem. 38 1413–1421. 10.1016/j.soilbio.2005.10.017 DOI

Tzelepis G., Karlsson M. (2021). “The fungal chitinases,” in Encyclopedia of Mycology, eds Zaragoza O., Casadevall A. (Amsterdam: Elsevier Science Publishing Co Inc; ), 23–31.

Uzarowicz Ł, Wolińska A., Błońska E., Szafranek-Nakonieczna A., Kuźniar A., Słodczyk Z., et al. (2020). Technogenic soils (Technosols) developed from mine spoils containing Fe sulphides: microbiological activity as an indicator of soil development following land reclamation. Appl. Soil Ecol. 156:103699. 10.1016/j.apsoil.2020.103699 DOI

Villeneuve P., Muderhwa J. M., Graille J., Haas M. J. (2000). Customizing lipases for biocatalysis: a survey of chemical, physical and molecular biological approaches. J. Mol. Catal. B Enzym. 9 113–148. 10.1016/S1381-1177(99)00107-1 DOI

Wang K., Ma X., Yin X., Wu C., Wang Z., Wu Y., et al. (2021). Difference and interplay of microbial communities, metabolic functions, trophic modes and influence factors between sludge and bulking agent in a composting matrix. Bioresour. Technol. 336:125085. 10.1016/j.biortech.2021.125085 PubMed DOI

Yuan J., Chadwick D., Zhang D., Li G., Chen S., Luo W., et al. (2016). Effects of aeration rate on maturity and gaseous emissions during sewage sludge composting. Waste Manag. 56 403–410. 10.1016/j.wasman.2016.07.017 PubMed DOI

Zhang B., Li G., Shen T., Wang J., Sun Z. (2000). Changes of microbial biomass C, N, and P and enzyme activities in soil incubated with the earthworms Metaphire guillelmi or Eisenia fetida. Soil Biol. Biochem. 32 2055–2062. 10.1016/S0038-0717(00)00111-5 DOI

Zhang Q., Hu J., Lee D. J., Chang Y., Lee Y. J. (2017). Sludge treatment: current research trends. Bioresour. Technol. 243 1159–1172. 10.1016/j.biortech.2017.07.070 PubMed DOI

Zheng M. M., Wang C., Li W. X., Guo L., Cai Z. J., Wang B. R., et al. (2021). Changes of acid and alkaline phosphatase activities in long-term chemical fertilization are driven by the similar soil properties and associated microbial community composition in acidic soil. Eur. J. Soil Biol. 104:103312. 10.1016/j.ejsobi.2021.103312 DOI

Zoglowek M., Lübeck P. S., Ahring B. K., Lübeck M. (2015). Heterologous expression of cellobiohydrolases in filamentous fungi - an update on the current challenges, achievements and perspectives. Process Biochem. 50 211–220. 10.1016/j.procbio.2014.12.018 DOI

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