Phylotype Dynamics of Bacterial P Utilization Genes in Microbialites and Bacterioplankton of a Monomictic Endorheic Lake
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27726035
DOI
10.1007/s00248-016-0862-1
PII: 10.1007/s00248-016-0862-1
Knihovny.cz E-resources
- Keywords
- DOP utilization, Extracellular enzymes, Microbial functional diversity, P turnover, Phylotype seasonality, Phytase,
- MeSH
- Alkaline Phosphatase genetics MeSH
- Bacteria enzymology genetics metabolism MeSH
- Bacteroidetes genetics metabolism MeSH
- Genes, Bacterial genetics MeSH
- RNA, Bacterial analysis MeSH
- DNA, Bacterial analysis MeSH
- Ecosystem MeSH
- Phosphorus chemistry metabolism MeSH
- Phylogeny * MeSH
- Genetic Variation * MeSH
- Genetic Markers genetics MeSH
- Lakes microbiology MeSH
- Oxygen chemistry MeSH
- Polymerase Chain Reaction methods MeSH
- Proteobacteria genetics metabolism MeSH
- Gene Expression Regulation, Bacterial MeSH
- Seasons MeSH
- Base Sequence MeSH
- Sequence Analysis MeSH
- Phosphorus Compounds chemistry metabolism MeSH
- Water chemistry MeSH
- Aquatic Organisms genetics metabolism MeSH
- Environment MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Mexico MeSH
- Names of Substances
- Alkaline Phosphatase MeSH
- RNA, Bacterial MeSH
- DNA, Bacterial MeSH
- Phosphorus MeSH
- Genetic Markers MeSH
- Oxygen MeSH
- Phosphorus Compounds MeSH
- Water MeSH
Microbes can modulate ecosystem function since they harbor a vast genetic potential for biogeochemical cycling. The spatial and temporal dynamics of this genetic diversity should be acknowledged to establish a link between ecosystem function and community structure. In this study, we analyzed the genetic diversity of bacterial phosphorus utilization genes in two microbial assemblages, microbialites and bacterioplankton of Lake Alchichica, a semiclosed (i.e., endorheic) system with marked seasonality that varies in nutrient conditions, temperature, dissolved oxygen, and water column stability. We focused on dissolved organic phosphorus (DOP) utilization gene dynamics during contrasting mixing and stratification periods. Bacterial alkaline phosphatases (phoX and phoD) and alkaline beta-propeller phytases (bpp) were surveyed. DOP utilization genes showed different dynamics evidenced by a marked change within an intra-annual period and a differential circadian pattern of expression. Although Lake Alchichica is a semiclosed system, this dynamic turnover of phylotypes (from lake circulation to stratification) points to a different potential of DOP utilization by the microbial communities within periods. DOP utilization gene dynamics was different among genetic markers and among assemblages (microbialite vs. bacterioplankton). As estimated by the system's P mass balance, P inputs and outputs were similar in magnitude (difference was <10 %). A theoretical estimation of water column P monoesters was used to calculate the potential P fraction that can be remineralized on an annual basis. Overall, bacterial groups including Proteobacteria (Alpha and Gamma) and Bacteroidetes seem to be key participants in DOP utilization responses.
Instituto de Geología Universidad Nacional Autónoma de México 04510 Mexico City Mexico
Posgrado en Ciencias Biológicas Universidad Nacional Autónoma de México 04510 Mexico City Mexico
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Appl Environ Microbiol. 2009 Mar;75(6):1508-16 PubMed
Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4474-9 PubMed
Protein Sci. 1996 Nov;5(11):2353-7 PubMed
Nucleic Acids Res. 2010 Jan;38(Database issue):D492-6 PubMed
J Mol Biol. 1982 May 5;157(1):105-32 PubMed
Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21219-23 PubMed
ISME J. 2009 May;3(5):563-72 PubMed
Nucleic Acids Res. 2015 Jan;43(Database issue):D222-6 PubMed
Science. 2012 Feb 10;335(6069):671-6 PubMed
MBio. 2013 Dec 31;5(1):e00744-13 PubMed
Arch Microbiol. 2006 Mar;185(1):1-13 PubMed
Water Res. 2014 Oct 1;62:229-40 PubMed
FEMS Microbiol Ecol. 2014 Nov;90(2):504-19 PubMed
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W29-37 PubMed
Nat Struct Biol. 2001 Jun;8(6):526-30 PubMed
Nucleic Acids Res. 2014 Jan;42(Database issue):D222-30 PubMed
ISME J. 2007 Aug;1(4):321-30 PubMed
J Mol Biol. 1990 Oct 5;215(3):403-10 PubMed
FEMS Microbiol Ecol. 2012 Dec;82(3):724-35 PubMed
Cell. 2001 Jun 29;105(7):817-20 PubMed
Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3458-63 PubMed
ISME J. 2013 Aug;7(8):1665-8 PubMed
Appl Environ Microbiol. 2009 Dec;75(23):7537-41 PubMed
Appl Environ Microbiol. 1996 Feb;62(2):316-22 PubMed
Biochemistry. 1974 Jan 15;13(2):211-22 PubMed
Syst Biol. 2010 May;59(3):307-21 PubMed
Microbes Environ. 2008;23(3):182-91 PubMed
