Predictive role BLVRA mRNA expression in hepatocellular cancer
Jazyk angličtina Země Mexiko Médium print
Typ dokumentu časopisecké články
PubMed
27740521
DOI
10.5604/16652681.1222104
PII: 1222104
Knihovny.cz E-zdroje
- MeSH
- hemoxygenasa-1 genetika MeSH
- hepatocelulární karcinom krev enzymologie genetika patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- membránové glykoproteiny genetika MeSH
- messenger RNA genetika MeSH
- nádorové biomarkery krev genetika MeSH
- nádory jater krev enzymologie genetika patologie MeSH
- NADPH-oxidasa 2 MeSH
- NADPH-oxidasy genetika MeSH
- oxidační stres genetika MeSH
- oxidoreduktasy působící na CH-CH vazby krev genetika MeSH
- progrese nemoci MeSH
- regulace genové exprese enzymů MeSH
- regulace genové exprese u nádorů MeSH
- senioři MeSH
- signální transdukce MeSH
- studie případů a kontrol MeSH
- upregulace MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biliverdin reductase MeSH Prohlížeč
- CYBA protein, human MeSH Prohlížeč
- CYBB protein, human MeSH Prohlížeč
- hemoxygenasa-1 MeSH
- HMOX1 protein, human MeSH Prohlížeč
- membránové glykoproteiny MeSH
- messenger RNA MeSH
- nádorové biomarkery MeSH
- NADPH-oxidasa 2 MeSH
- NADPH-oxidasy MeSH
- oxidoreduktasy působící na CH-CH vazby MeSH
UNLABELLED: Introduction and aim. Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor. It is primarily caused by hepatic cirrhosis or chronic viral hepatitis. Hepatic carcinogenesis is associated with increased oxidative stress. Thus, the aim of our study was to assess expression of the genes involved in the homeostasis of oxidative stress in patients with HCC. MATERIAL AND METHODS: The study was performed on 32 patients with primary HCC (verified by liver histology in 29 patients) and 27 control subjects (in 11 subjects, liver histology was available either with no or minimal changes in the liver tissue). Gene expressions of heme oxygenase 1 (HMOX1), biliverdin reductase A/B (BLVRA/B), NADPH oxidase 2 (NOX2) and p22phox were analyzed in the liver and peripheral blood leukocytes (PBL) in the subjects. RESULTS: Compared to controls, almost a 3 times higher mRNA level of BLVRA was detected in livers of HCC patients (p = 0.002); while those of BLVRB as well as HMOX1 were unchanged (p > 0.05). In accord with these results in the liver tissue, BLVRA mRNA levels in PBL were also significantly increased in HCC patients (p = 0.012). mRNA levels of NOX2 and p22phox in the liver tissue, although higher in HCC patients, did not differ significantly compared to control subjects (p > 0.05). Nevertheless, NOX2 mRNA level in PBL was significantly higher in HCC patients (p = 0.003). CONCLUSIONS: BLVRA mRNA levels in the liver as well as in PBL are significantly higher in HCC patients most likely as a feedback mechanism to control increased oxidative stress associated with HCC progression.
Citace poskytuje Crossref.org
