Predictive role BLVRA mRNA expression in hepatocellular cancer
Language English Country Mexico Media print
Document type Journal Article
PubMed
27740521
DOI
10.5604/16652681.1222104
PII: 1222104
Knihovny.cz E-resources
- MeSH
- Heme Oxygenase-1 genetics MeSH
- Carcinoma, Hepatocellular blood enzymology genetics pathology MeSH
- Middle Aged MeSH
- Humans MeSH
- Membrane Glycoproteins genetics MeSH
- RNA, Messenger genetics MeSH
- Biomarkers, Tumor blood genetics MeSH
- Liver Neoplasms blood enzymology genetics pathology MeSH
- NADPH Oxidase 2 MeSH
- NADPH Oxidases genetics MeSH
- Oxidative Stress genetics MeSH
- Oxidoreductases Acting on CH-CH Group Donors blood genetics MeSH
- Disease Progression MeSH
- Gene Expression Regulation, Enzymologic MeSH
- Gene Expression Regulation, Neoplastic MeSH
- Aged MeSH
- Signal Transduction MeSH
- Case-Control Studies MeSH
- Up-Regulation MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- biliverdin reductase MeSH Browser
- CYBA protein, human MeSH Browser
- CYBB protein, human MeSH Browser
- Heme Oxygenase-1 MeSH
- HMOX1 protein, human MeSH Browser
- Membrane Glycoproteins MeSH
- RNA, Messenger MeSH
- Biomarkers, Tumor MeSH
- NADPH Oxidase 2 MeSH
- NADPH Oxidases MeSH
- Oxidoreductases Acting on CH-CH Group Donors MeSH
UNLABELLED: Introduction and aim. Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor. It is primarily caused by hepatic cirrhosis or chronic viral hepatitis. Hepatic carcinogenesis is associated with increased oxidative stress. Thus, the aim of our study was to assess expression of the genes involved in the homeostasis of oxidative stress in patients with HCC. MATERIAL AND METHODS: The study was performed on 32 patients with primary HCC (verified by liver histology in 29 patients) and 27 control subjects (in 11 subjects, liver histology was available either with no or minimal changes in the liver tissue). Gene expressions of heme oxygenase 1 (HMOX1), biliverdin reductase A/B (BLVRA/B), NADPH oxidase 2 (NOX2) and p22phox were analyzed in the liver and peripheral blood leukocytes (PBL) in the subjects. RESULTS: Compared to controls, almost a 3 times higher mRNA level of BLVRA was detected in livers of HCC patients (p = 0.002); while those of BLVRB as well as HMOX1 were unchanged (p > 0.05). In accord with these results in the liver tissue, BLVRA mRNA levels in PBL were also significantly increased in HCC patients (p = 0.012). mRNA levels of NOX2 and p22phox in the liver tissue, although higher in HCC patients, did not differ significantly compared to control subjects (p > 0.05). Nevertheless, NOX2 mRNA level in PBL was significantly higher in HCC patients (p = 0.003). CONCLUSIONS: BLVRA mRNA levels in the liver as well as in PBL are significantly higher in HCC patients most likely as a feedback mechanism to control increased oxidative stress associated with HCC progression.
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