Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
27860531
DOI
10.1089/mdr.2016.0159
Knihovny.cz E-resources
- Keywords
- Clostridium difficile, MLVA, PCR ribotype 001, Thr82Ile, antimicrobial drug resistance, capillary electrophoresis ribotyping,
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Tertiary Care Centers MeSH
- Ciprofloxacin pharmacology MeSH
- Clostridioides difficile classification drug effects genetics isolation & purification MeSH
- DNA, Bacterial genetics metabolism MeSH
- DNA Gyrase genetics metabolism MeSH
- Electrophoresis, Capillary MeSH
- Gene Expression MeSH
- Fluoroquinolones pharmacology MeSH
- Clindamycin pharmacology MeSH
- Clostridium Infections drug therapy microbiology MeSH
- Humans MeSH
- Methyltransferases genetics metabolism MeSH
- Metronidazole pharmacology MeSH
- Microbial Sensitivity Tests MeSH
- Minisatellite Repeats MeSH
- Drug Resistance, Multiple, Bacterial genetics MeSH
- Moxifloxacin MeSH
- Multilocus Sequence Typing MeSH
- Retrospective Studies MeSH
- Ribotyping MeSH
- Aged MeSH
- Amino Acid Substitution MeSH
- Vancomycin pharmacology MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Ciprofloxacin MeSH
- DNA, Bacterial MeSH
- DNA Gyrase MeSH
- Fluoroquinolones MeSH
- Clindamycin MeSH
- Methyltransferases MeSH
- Metronidazole MeSH
- Moxifloxacin MeSH
- rRNA (adenosine-O-2'-)methyltransferase MeSH Browser
- Vancomycin MeSH
AIM: To perform a retrospective analysis of the high occurrence of Clostridium difficile infection in the surgical department of a Czech tertiary care hospital and to identify weaknesses in C. difficile infection (CDI) prevention and control policies. METHODS: Clinical and epidemiological data on eleven CDI cases were collected. C. difficile isolates were characterized by capillary electrophoresis ribotyping, multilocus variable tandem repeat analysis (MLVA), gyrA gene fragment sequencing, and erm(B) fragment PCR amplification. Antibiotic susceptibility to metronidazole, vancomycin, ciprofloxacin, moxifloxacin, and clindamycin was tested. FINDINGS: Eleven CDI cases were caused by C. difficile PCR ribotype 001 strains. These strains revealed two different MLVA profiles with 11 tandem repeat differences. All isolates were susceptible to metronidazole and vancomycin and resistant to ciprofloxacin (MIC ≥32 mg/L), moxifloxacin (MIC ≥32 mg/L), and clindamycin (MIC ≥256 mg/L). All isolates revealed amino acid substitution Thr82Ile, in the GyrA and were erm(B) negative. CONCLUSION: Two fluoroquinolone and clindamycin-resistant C. difficile PCR ribotype 001 strain clusters occurred at one of the surgical departments of a tertiary care hospital. Ineffective decontamination with suboptimal concentration and time of exposure of sporicidal disinfectants may have resulted in C. difficile transmission.
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