Actinobacillus pleuropneumoniae causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of A. pleuropneumoniae that were serologically distinct from the previously characterized 15 serovars were described, and a proposal was put forward that they comprised a new serovar, serovar 16. Here we used whole-genome sequencing of the proposed serovar 16 reference strain A-85/14 to confirm the presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of strain A-85/14, and a PCR was formulated that differentiated serovar 16 isolates from all 15 known serovars and other common respiratory pathogenic/commensal bacteria of pigs. Analysis of the capsule locus of strain A-85/14 combined with the previous serological data show the existence of a sixteenth serovar-designated serovar 16-of A. pleuropneumoniae.

Department of Microbiology and Infection Control Statens Serum Institut Copenhagen Denmark

Department of Microbiology and Infectious Diseases University of Veterinary Medicine Budapest Hungary

Department of Pathology and Pathogen Biology The Royal Veterinary College Hawkshead Campus Hatfield United Kingdom

Groupe de Recherche sur les Maladies Infectieuses du Porc Faculté de Médecine Vétérinaire Université de Montréal Montréal Québec Canada

Section of Paediatrics Department of Medicine Imperial College London St Mary's Campus London United Kingdom

Veterinary Research Institute Brno Czech Republic

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