Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

Department of Microbiology and Infection Control Statens Serum Institut Copenhagen Denmark

Department of Microbiology and Infectious Diseases University of Veterinary Medicine Budapest Hungary

Department of Pathology and Pathogen Biology The Royal Veterinary College Hawkshead Campus UK

Department of Veterinary Medicine University of Cambridge Cambridge UK

Faculty of Infectious and Tropical Diseases London School of Hygiene and Tropical Medicine London UK

Groupe de Recherche sur les Maladies Infectieuses du Porc Faculté de Médecine Vétérinaire Université de Montréal Québec Canada

OVISLAB S L Barcelona Spain

Section of Paediatrics Department of Medicine Imperial College London St Mary's Campus London UK

The Wellcome Trust Sanger Institute Hinxton UK

Veterinary Research Institute Hudcova 70 621 00 Brno Czech Republic

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