A Review of Methods for Detection of Hepatozoon Infection in Carnivores and Arthropod Vectors
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, přehledy, práce podpořená grantem
PubMed
28055571
DOI
10.1089/vbz.2016.1963
Knihovny.cz E-zdroje
- Klíčová slova
- Hepatozoon, PCR, diagnostics,
- MeSH
- Carnivora parazitologie MeSH
- členovci - vektory parazitologie MeSH
- Eucoccidiida genetika izolace a purifikace MeSH
- polymerázová řetězová reakce metody veterinární MeSH
- protozoální infekce zvířat diagnóza parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Vector-borne protists of the genus Hepatozoon belong to the apicomplexan suborder Adeleorina. The taxonomy of Hepatozoon is unsettled and different phylogenetic clades probably represent evolutionary units deserving the status of separate genera. Throughout our review, we focus on the monophyletic assemblage of Hepatozoon spp. from carnivores, classified as Hepatozoon sensu stricto that includes important pathogens of domestic and free-ranging canine and feline hosts. We provide an overview of diagnostic methods and approaches from classical detection in biological materials, through serological tests to nucleic acid amplification tests (NAATs). Critical review of used primers for the 18S rDNA is provided, together with information on individual primer pairs. Extension of used NAATs target to cover also mitochondrial genes is suggested as a key step in understanding the diversity and molecular epidemiology of Hepatozoon infections in mammals.
Biology Centre Institute of Parasitology Czech Academy of Sciences České Budějovice Czech Republic
CEITEC VFU University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic
Department for Bacteriology and Parasitology Croatian Veterinary Institute Zagreb Croatia
Koret School of Veterinary Medicine Hebrew University Rehovot Israel
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