Expression and distribution of extensins and AGPs in susceptible and resistant banana cultivars in response to wounding and Fusarium oxysporum
Language English Country Great Britain, England Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
28218299
PubMed Central
PMC5316987
DOI
10.1038/srep42400
PII: srep42400
Knihovny.cz E-resources
- MeSH
- Musa microbiology physiology MeSH
- Epitopes immunology MeSH
- Fusarium * MeSH
- Glycoproteins genetics metabolism MeSH
- Host-Pathogen Interactions genetics MeSH
- Plant Roots genetics metabolism microbiology MeSH
- Mucoproteins genetics metabolism MeSH
- Plant Diseases genetics microbiology MeSH
- Disease Resistance genetics MeSH
- Gene Expression Regulation, Plant * MeSH
- Plant Proteins genetics immunology metabolism MeSH
- Protein Transport MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- arabinogalactan proteins MeSH Browser
- Epitopes MeSH
- extensin protein, plant MeSH Browser
- Glycoproteins MeSH
- Mucoproteins MeSH
- Plant Proteins MeSH
Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is soil-borne disease of banana (Musa spp.) causing significant economic losses. Extensins and arabinogalactan proteins (AGPs) are cell wall components important for pathogen defence. Their significance for Foc resistance in banana was not reported so far. In this study, two banana cultivars differing in Foc sensitivity were used to monitor the changes in transcript levels, abundance and distribution of extensins and AGPs after wounding and Foc inoculation. Extensins mainly appeared in the root cap and meristematic cells. AGPs recognized by JIM13, JIM8, PN16.4B4 and CCRC-M134 antibodies located in root hairs, xylem and root cap. Individual AGPs and extensins showed specific radial distribution in banana roots. At the transcript level, seven extensins and 23 AGPs were differentially expressed between two banana cultivars before and after treatments. Two extensins and five AGPs responded to the treatments at the protein level. Most extensins and AGPs were up-regulated by wounding and pathogen inoculation of intact plants but down-regulated by pathogen attack of wounded plants. Main components responsible for the resistance of banana were MaELP-2 and MaPELP-2. Our data revealed that AGPs and extensins represent dynamic cell wall components involved in wounding and Foc resistance.
College of Horticulture South China Agricultural University Guangzhou 510642 China
Institute of Biotechnology Guangxi Academy of Agricultural Sciences Nanning 530007 China
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