Host specificity and basic ecology of Mammomonogamus (Nematoda, Syngamidae) from lowland gorillas and forest elephants in Central African Republic
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
28274296
DOI
10.1017/s0031182017000221
PII: S0031182017000221
Knihovny.cz E-resources
- Keywords
- Gorilla gorilla gorilla, Loxodonta cyclotis, cox1, 18S rDNA, Dzanga-Sangha Protected Areas, haplotype, interspecies transmission,
- MeSH
- DNA, Helminth genetics MeSH
- Phylogeny MeSH
- Gorilla gorilla * MeSH
- Host Specificity MeSH
- Strongylida Infections epidemiology parasitology veterinary MeSH
- Host-Parasite Interactions MeSH
- Ape Diseases epidemiology parasitology MeSH
- Prevalence MeSH
- Helminth Proteins genetics MeSH
- Electron Transport Complex IV genetics MeSH
- RNA, Ribosomal, 18S genetics MeSH
- Sequence Analysis, DNA veterinary MeSH
- Elephants * MeSH
- Strongyloidea classification genetics physiology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Central African Republic epidemiology MeSH
- Names of Substances
- DNA, Helminth MeSH
- Helminth Proteins MeSH
- Electron Transport Complex IV MeSH
- RNA, Ribosomal, 18S MeSH
Syngamid strongylids of the genus Mammomonogamus undoubtedly belong among the least known nematodes with apparent zoonotic potential and the real diversity of the genus remains hard to evaluate without extensive molecular data. Eggs of Mammomonogamus sp. are frequently found in feces of African forest elephants (Loxodonta cyclotis) and western lowland gorillas (Gorilla gorilla gorilla) in Dzanga-Sangha Protected Areas. Using sedimentation-based coproscopic techniques, we found the eggs of Mammomonogamus in 19·7% elephant and 54·1% gorilla fecal samples with 8-55 and 1-24 eggs per gram of fecal sediment for elephants and gorillas, respectively. We used a combination of light microscopy, scanning electron microscopy and analysis of cytochrome c oxidase subunit I (cox1) and a partial sequence of 18S rDNA isolated from single eggs to test the hypothesis of possible Mammomonogamus conspecificity in gorillas and elephants. Whereas 18S rDNA sequences were identical in both gorillas and elephants, we distinguished seven different haplotypes within the cox1. Two haplotypes were found in both gorillas and elephants suggesting sharing of Mammomonogamus. Assignment of the parasite to M. loxodontis is proposed. Provided sequences represent the first genomic data on Mammomonogamus spp.
Institute of Vertebrate Biology Czech Academy of Sciences Květná 8 603 65 Brno Czech Republic
The Wildlife Conservation Society BP 1053 Bangui Central African Republic
WWF Dzanga Sangha Protected Areas BP 1053 Bangui Central African Republic
References provided by Crossref.org
Diversity of Mammomonogamus (Nematoda: Syngamidae) in large African herbivores
