RATIONALE: Many kinds of nanoparticles (NPs) have been used for mass spectrometry (MS) so far. Here we report the first use of flower-like gold nanoparticles (AuNPs) as a mediator to enhance ionization in MS of peptides and proteins. METHODS: Flower-like AuNPs were characterized using transmission and scanning electron microscopy, UV-VIS spectrophotometry, and laser desorption/ionization (LDI)-MS and compared with polyhedral AuNPs. Mass spectra were obtained in positive ion mode using a time-of-flight (TOF) analyzer coupled with either matrix-assisted laser desorption/ionization (MALDI) or surface-assisted laser desorption/ionization (SALDI) methods. RESULTS: The intensities of peptide peaks (m/z 500-3500) were up to 7.5× and up to 7× higher using flower-like AuNPs and flower-like AuNPs-enriched α-cyano-4-hydroxycinnamic acid (CHCA) matrix respectively, than the classical CHCA matrix. The signals of higher mass peptide/protein peaks (m/z 3600-17000) were up to 2× higher with using flower-like AuNPs-enriched CHCA matrix than conventional CHCA matrix. The signal of profile peaks generated by intact cell MALDI-TOFMS of fibroblast suspension (m/z 4000-20000) was 2× higher with using flower-like AuNPs combined with sinapinic acid (SA) compared to SA matrix alone. The use of flower-like AuNPs as internal calibration standard for the calibration of MS spectra of peptides was performed. CONCLUSIONS: Flower-like AuNPs and flower-like AuNPs combined with CHCA or SA as combined matrices for MS measurement of peptides and proteins were used. Comparison of the conventional MALDI method and our method with flower-like AuNPs was carried out. In addition, gold clusters generated from flower-like AuNPs by SALDI provide a suitable internal calibration standard for MS analysis of peptides. Copyright © 2015 John Wiley & Sons, Ltd.

Department of Chemistry Faculty of Science Masaryk University Kamenice 5 A14 62500 Brno Czech Republic

Department of Histology and Embryology Faculty of Medicine Masaryk University Kamenice 3 A1 62500 Brno Czech Republic

Citace poskytuje Crossref.org

Matrix enrichment by black phosphorus improves ionization and reproducibility of mass spectrometry of intact cells, peptides, and amino acids

Laser ablation synthesis of metal-doped gold clusters from composites of gold nanoparticles with metal organic frameworks

Clusters of Monoisotopic Elements for Calibration in (TOF) Mass Spectrometry

Laser Desorption Ionization Quadrupole Ion Trap Time-of-Flight Mass Spectrometry of Au m Fe n+/- Clusters Generated from Gold-Iron Nanoparticles and their Giant Nanoflowers. Electrochemical and/or Plasma Assisted Synthesis