The involvement of sirtuin 1 and heme oxygenase 1 in the hepatoprotective effects of quercetin against carbon tetrachloride-induced sub-chronic liver toxicity in rats
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28347707
DOI
10.1016/j.cbi.2017.03.014
PII: S0009-2797(17)30062-5
Knihovny.cz E-zdroje
- Klíčová slova
- Carbon tetrachloride, Heme oxygenase 1, Hepatoprotection, Hepatotoxicity, Quercetin, Sirtuin 1,
- MeSH
- alanintransaminasa krev MeSH
- aspartátaminotransferasy krev MeSH
- bilirubin krev MeSH
- down regulace účinky léků MeSH
- hemoxygenasa-1 metabolismus MeSH
- imunohistochemie MeSH
- játra metabolismus patologie MeSH
- krysa rodu Rattus MeSH
- ochranné látky farmakologie MeSH
- otrava chloridem uhličitým metabolismus patologie prevence a kontrola MeSH
- potkani Wistar MeSH
- quercetin farmakologie MeSH
- sirtuin 1 metabolismus MeSH
- upregulace účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alanintransaminasa MeSH
- aspartátaminotransferasy MeSH
- bilirubin MeSH
- hemoxygenasa-1 MeSH
- ochranné látky MeSH
- quercetin MeSH
- sirtuin 1 MeSH
The present study was designed to evaluate the therapeutic potential of quercetin in a sub-chronic model of hepatotoxicity. The roles of putative antioxidant enzymes, sirtuin 1 (SIRT1) and heme oxygenase 1 (HO-1), in hepatoprotection were also addressed. Sub-chronic liver injury was induced in rats by intraperitoneal administration of 0.5 ml/kg carbon tetrachloride (CTC), once every 3 days, for 2 weeks. Some CTC rats were concurrently treated with 100 mg/kg quercetin, intragastrically, once every day, for 2 weeks. The effects of these drugs in the liver were evaluated by biochemical, histological, immunohistochemical and molecular biological studies. CTC triggered oxidative damage to the liver as unanimously shown by altered biochemical parameters and liver morphology. Furthermore, CTC highly upregulated HO-1 and SIRT1 expression levels. Concomitant treatment of rats with quercetin downregulated SIRT1 expression and ameliorated the hepatotoxic effects of CTC. However, quercetin did not have any significant effect on HO-1 expression and bilirubin levels. Collectively, these results suggest that the antioxidant and cytoprotective effects of quercetin in CTC treated rats were SIRT1 mediated and less dependent on HO-1. Thus, pharmacologic modulation of SIRT1 could provide a logic therapeutic approach in sub-chronic hepatotoxicity.
Citace poskytuje Crossref.org
SIRT1 Modulators in Experimentally Induced Liver Injury
