Mycobacterium avium paratuberculosis (Map) is a pathogen which causes a chronic progressive granulomatous enteritis known as paratuberculosis or Johne's disease and it primarily affects wild and domestic ruminants. The aim of this research was to examine a flock which consisted of 294 goats and was located in Garfagnana district (Tuscany, Italy) performing ELISA tests, culture and IS900 PCR assay; direct diagnostic methods were carried out not only on bulk tank milk and cheese samples but also on individual milk and tissue specimens collected from nine subjects positive to ELISA tests. Out of 294 animals, 20 goats (6.8%) were positive to ELISA surveys. Bulk tank milk samples were negative to culture and to PCR assay carried out on the DNA extracted directly from them, while, with respect to cheese, Map was detected by culture in 2/12 (16.66%) cheeses ripened for 3-7 days, and by PCR in 2/12 (16.66%) cheeses ripened for 3-7 days and in 3/12 (25%) cheeses ripened for 45 days. Regarding individual milk samples, Map was detected by culture in 2/9 (22.22%) specimens and by PCR in 5/9 (55.55%) samples. Furthermore, Map was isolated from the intestine in 9/9 (100%) animals, from the mesenteric lymph nodes in 8/9 (88.88%) subjects, from the liver in 4/9 (44.44%) goats, from the spleen in 5/9 (55.55%) animals, while Map DNA was found in all the tissue samples analyzed.The results demonstrated the presence of paratuberculosis in a goat flock located in Garfagnana district (Tuscany, Italy).

Azienda U S L 2 Lucca Italy

Department of Veterinary Sciences University of Pisa Viale delle Piagge 2 Pisa Italy

Zobrazit více v PubMed

Appl Environ Microbiol. 2002 May;68(5):2428-35 PubMed

Trop Anim Health Prod. 2015 Dec;47(8):1567-71 PubMed

Vet Microbiol. 2001 Mar 20;79(2):123-31 PubMed

J Clin Microbiol. 1999 Apr;37(4):1077-83 PubMed

Foodborne Pathog Dis. 2010 Apr;7(4):351-5 PubMed

Vet Microbiol. 2003 Mar 20;92(1-2):135-43 PubMed

J Clin Microbiol. 2002 Dec;40(12):4760-2 PubMed

Int J Food Microbiol. 2016 Jan 18;217:195-9 PubMed

J Vet Diagn Invest. 1993 Oct;5(4):629-31 PubMed

Vet Microbiol. 2000 Dec 20;77(3-4):309-24 PubMed

Can J Vet Res. 2001 Oct;65(4):201-5 PubMed

Crit Rev Microbiol. 2012 Feb;38(1):52-93 PubMed

Int J Food Microbiol. 2010 Jan 31;137(1):32-9 PubMed

Int J Food Microbiol. 2010 Jul 31;141 Suppl 1:S87-90 PubMed

Front Microbiol. 2017 Jan 04;7:2112 PubMed

Appl Environ Microbiol. 1998 Mar;64(3):999-1005 PubMed

Front Immunol. 2015 Mar 04;6:96 PubMed

J Appl Microbiol. 2012 Nov;113(5):1014-26 PubMed

J Dairy Sci. 2003 Dec;86(12):3849-56 PubMed

Vet Microbiol. 2006 Mar 10;113(1-2):13-24 PubMed

Aust Vet J. 2003 Jan-Feb;81(1-2):71-5 PubMed

BMC Vet Res. 2011 Sep 19;7:54 PubMed

Rev Argent Microbiol. 2012 Jul-Sep;44(3):201-15 PubMed

Vet Microbiol. 2008 Mar 18;127(3-4):300-8 PubMed

Appl Environ Microbiol. 2005 Dec;71(12):8934-6 PubMed

Res Vet Sci. 2013 Apr;94(2):191-7 PubMed

Vet Microbiol. 2015 Jun 12;177(3-4):403-8 PubMed

J Clin Microbiol. 2005 Aug;43(8):3704-12 PubMed

New Microbiol. 2002 Apr;25(2):131-7 PubMed

Appl Environ Microbiol. 2005 Jun;71(6):2853-61 PubMed

J Clin Microbiol. 2006 Mar;44(3):1081-3 PubMed

Vet Microbiol. 2006 Aug 25;116(1-3):129-37 PubMed

Appl Environ Microbiol. 2009 Jan;75(2):414-8 PubMed

Vet Res Commun. 2000 Nov;24(7):435-43 PubMed

Can Vet J. 2006 Sep;47(9):874-82 PubMed

BMC Vet Res. 2015 Mar 24;11:74 PubMed

Vet Microbiol. 2006 Jun 15;115(1-3):91-101 PubMed