BACKGROUND AND AIMS: Understanding the direct consequences of polyploidization is necessary for assessing the evolutionary significance of this mode of speciation. Previous studies have not studied the degree of between-population variation that occurs due to these effects. Although it is assumed that the effects of the substances that create synthetic polyploids disappear in second-generation synthetic polyploids, this has not been tested. METHODS: The direct consequences of polyploidization were assessed and separated from the effects of subsequent evolution in Vicia cracca , a naturally occurring species with diploid and autotetraploid cytotypes. Synthetic tetraploids were created from diploids of four mixed-ploidy populations. Performance of natural diploids and tetraploids was compared with that of synthetic tetraploids. Diploid offspring of the synthetic tetraploid mothers were also included in the comparison. In this way, the effects of colchicine application in the maternal generation on offspring performance could be compared independently of the effects of polyploidization. KEY RESULTS: The sizes of seeds and stomata were primarily affected by cytotype, while plant performance differed between natural and synthetic polyploids. Most performance traits were also determined by colchicine application to the mothers, and most of these results were largely population specific. CONCLUSIONS: Because the consequences of colchicine application are still apparent in the second generation of the plants, at least the third-generation polyploids should be considered in future comparisons. The specificities of the colchicine-treated plants may also be caused by strong selection pressures during the creation of synthetic polyploids. This could be tested by comparing the initial sizes of plants that survived the colchicine treatments with those of plants that did not. High variation between populations also suggests that different polyploids follow different evolutionary trajectories, and this should be considered when studying the effects of polyploidization.

Department of Botany Faculty of Science Charles University Prague Czech Republic

Institute of Botany Academy of Sciences of the Czech Republic Průhonice Czech Republic

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Amiri S, Kazemitabaar S, Ranjbar K, Azadbakht M.. 2010. The effect of trifluralin and colchicine treatments on morphological characteristics of jimsonweed. Trakia Journal of Sciences 8: 47–61.

Asker S. 1980. Gametophytic apomixis – elements and genetic regulation. Hereditas 93: 277–293.

Asker S, Jerling L.. 1992. Apomixis in plants. Boca Raton, FL: CRC Press.

Balao F, Herrera J, Talavera S.. 2011. Phenotypic consequences of polyploidy and genome size at the microevolutionary scale: a multivariate morphological approach. New Phytologist, 192: 256–265. PubMed

Benard RB, Toft CA.. 2007. Effect of seed size on seedling performance in a long-lived desert perennial shrub (

Benard RB, Toft CA.. 2008. Fine-scale spatial heterogeneity and seed size determine early seedling survival in a desert perennial shrub (

Bennett M, Finch R, Barclay I.. 1976. The time rate and mechanism of chromosome elimination in

ter Braak C, Šmilauer P.. 1998. Canoco reference manual and users guide to Canoco forWindows: Software for canonical community ordination (version 4). Ithaca: Microcomputer Power.

Bretagnolle F, Lumaret R.. 1995. Bilateral polyploidization in

Bretagnolle F, Thompson JD, Lumaret R.. 1995. The influence of seed size variation on seed germination and seedling vigor in diploid and tetraploid

Castro S, Loureiro J, Prochazka T, Münzbergová Z.. 2012. Cytotype distribution at a diploid–hexaploid contact zone in PubMed PMC

Černá L, Münzbergová Z.. 2013. Comparative population dynamics of two closely related species differing in ploidy level. PLoS One 8: e75563. doi: 10.1371/journal.pone.0075563. PubMed PMC

Cohen H, Fait A, Tel-Zur N.. 2013. Morphological, cytological and metabolic consequences of autopolyploidization in PubMed PMC

Comai L. 2005. The advantages and disadvantages of being polyploid. Nature Reviews Genetics 6: 836–846. PubMed

Contreras RN, Ranney TG, Tallury SP.. 2007. Reproductive behavior of diploid and allotetraploid

Dar TH, Raina SN, Goel S.. 2013. Molecular analysis of genomic changes in synthetic autotetraploid

Dudits D, Torok K, Cseri A, et al. 2016. Response of organ structure and physiology to autotetraploidization in early development of energy willow PubMed PMC

Eliášová A, Münzbergová Z.. 2014. Higher seed size and germination rate may favour autotetraploids of

Eliášová A, Münzbergová Z.. 2017. Factors influencing distribution and local coexistence of diploids and tetraploids of PubMed

Eliášová A, Trávníček P, Mandák B, Münzbergová Z.. 2014. Autotetraploids of PubMed PMC

Ellis MM. 2013. Evidence for transient dynamics in plant populations based on long-term demographic data. Journal of Ecology 101: 734–742.

Francis A, Jones RN, Parker JS, Posselt UK.. 1990. Colchicine-induced heritable variation in cell-size and chloroplast numbers in leaf mesophyll-cells of diploid ryegrass (

Froelicher Y, Bassene JB, Jedidi-Neji E, et al. 2007. Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets. Plant Cell Reports 26: 937–944. PubMed

Gao R, Wang HB, Dong B, et al. 2016. Morphological, genome and gene expression changes in newly induced autopolyploid PubMed PMC

Griffin AR, Vuong TD, Vaillancourt RE, et al. 2012. The breeding systems of diploid and neoautotetraploid clones of PubMed

Hassan L, Jones RN.. 1994. Long-range effects of colchicine sensitivity on meiosis in

Hassan L, Wazuddin M.. 2000. Colchicine-induced variation of cell size and chloroplast number in leaf mesophyll of rice. Plant Breeding 119: 531–533.

Hegarty M, Coate J, Sherman-Broyles S, Abbott R, Hiscock S, Doyle J.. 2013. Lessons from natural and artificial polyploids in higher plants. Cytogenetic and Genome Research 140: 204–225. PubMed

Hosseini H, Chehrazi M, Sorestani M, Ahmadi D.. 2013. Polyploidy and comparison of diploid and autotetraploid seedling of Madagascar periwinkle (

Hoya A, Shibaike H, Morita T, Ito M.. 2007. Germination characteristics of native Japanese dandelion autopolyploids and their putative diploid parent species. Journal of Plant Research 120: 139–147. PubMed

Husband BC, Schemske DW.. 2000. Ecological mechanisms of reproductive isolation between diploid and tetraploid

Husband BC, Ozimec B, Martin SL, Pollock L.. 2008. Mating consequences of polyploid evolution in flowering plants: current trends and insights from synthetic polyploids. International Journal of Plant Sciences 169: 195–206.

Husband BC, Baldwin SJ, Sabara HA.. 2016. Direct vs. indirect effects of whole-genome duplication on prezygotic isolation in PubMed

Jaskani MJ, Kwon SW, Kim DH.. 2005. Comparative study on vegetative, reproductive and qualitative traits of seven diploid and tetraploid watermelon lines. Euphytica 145: 259–268.

Jones GH, Khazanehdari KA, FordLloyd BV.. 1996. Meiosis in the leek (

Krahulcová A, Krahulec F.. 2000. Offspring diversity in

Krahulcová A, Papoušková S, Krahulec F.. 2004. Reproduction mode in the allopolyploid facultatively apomictic hawkweed PubMed

Laurie DA, Bennett MD.. 1989. The timing of chromosome elimination in hexaploid wheat × maize crosses. Genome 32: 953–961.

Lepš J, Šmilauer P.. 2003. Multivariate analysis of ecological data using CANOCO. Cambridge: Cambridge University Press.

Levin D. 1983. Polyploidy and novelty in flowering plants. American Naturalist 122: 1–25.

Levin D. 2002. The role of chromosomal change in plant evolution. Oxford: Oxford University Press.

Lignowski EM, Scott EG.. 1972. Effect of trifluralin on mitosis. Weed Science 20: 267–270.

Loidl J. 1995. Meiotic chromosome-pairing in triploid and tetraploid PubMed PMC

Lumaret R. 1988. Adaptive strategies and ploidy levels. Acta Oecologica-Oecologia Plantarum 9: 83–93.

Maceira NO, Jacquard P, Lumaret R.. 1993. Competition between diploid and derivative autotetraploid PubMed

Madon M, Clyde MM, Hasim H, Yusuf M, Mat H, Saratha S.. 2005. Polyploidy induction of oil palm through colchicine and oryzalin treatments. Journal of Oil Palm Research 17: 110–123.

Maherali H, Walden AE, Husband BC.. 2009. Genome duplication and the evolution of physiological responses to water stress. New Phytologist 184: 721–731. PubMed

Mandáková T, Münzbergová Z.. 2008. Morphometric and genetic differentiation of diploid and hexaploid populations of

Matsushita SC, Tyagi AP, Thornton GM, Pires JC, Madlung A.. 2012. Allopolyploidization lays the foundation for evolution of distinct populations: evidence from analysis of synthetic PubMed PMC

Matter P, Kettle CJ, Ghazoul J, Hahn T, Pluess AR.. 2013. Evaluating contemporary pollen dispersal in two common grassland species PubMed

McCollum GD. 1958. Comparative studies of chromosome pairing in natural and induced tetraploid PubMed

Meimberg H, Rice KJ, Milan NF, Njoku CC, McKay JK.. 2009. Multiple origins promote the ecological amplitude of allopolyploid PubMed

Münzbergová Z. 2007. No effect of ploidy level in plant response to competition in a common garden experiment. Biological Journal of the Linnean Society 92: 211–219.

Münzbergová Z. 2006. Ploidy level interacts with population size and habitat conditions to determine the degree of herbivory damage in plant populations. Oikos 115: 443–452.

Münzbergová Z, Plačková I.. 2010. Seed mass and population characteristics interact to determine performance of

Oswald BP, Nuismer SL.. 2011. Neopolyploidy and diversification in PubMed PMC

Otto SP. 2007. The evolutionary consequences of polyploidy. Cell 131: 452–462. PubMed

Otto SP, Whitton J.. 2000. Polyploid incidence and evolution. Annual Review of Genetics 34: 401–437. PubMed

Pavlíková Z, Paštová L, Münzbergová Z.. 2017. Synthetic polyploids in

Ramsey J, Ramsey TS.. 2014. Ecological studies of polyploidy in the 100 years following its discovery. Philosophical Transactions of the Royal Society B: Biological Sciences 369: pii: 20130352. doi:10.1098/rstb.2013.0352.20. PubMed PMC

Ramsey J, Schemske DW.. 2002. Neopolyploidy in flowering plants. Annual Review of Ecology and Systematics 33: 589–639.

R Development Core Team. 2011. R: a language and environment for statistical computing

Riddle NC, Birchler JA.. 2008. Comparative analysis of inbred and hybrid maize at the diploid and tetraploid levels. Theoretical and Applied Genetics 116: 563–576. PubMed

Riddle NC, Kato A, Birchler JA.. 2006. Genetic variation for the response to ploidy change in PubMed

Sanchez-Moran E, Benavente E, Orellana J.. 2001. Analysis of karyotypic stability of homoeologous-pairing (ph) mutants in allopolyploid wheats. Chromosoma 110: 371–377. PubMed

Segraves KA, Thompson JN.. 1999. Plant polyploidy and pollination: floral traits and insect visits to diploid and tetraploid PubMed

Semeniuk P, Arisumi T.. 1968. Colchicine-induced tetraploid and cytochimeral roses. Botanical Gazette, 129: 190–193.

Singsit C, Hanneman RE.. 1991. Haploid induction in Mexican polyploid species and colchicine-doubled derivatives. American Potato Journal 68: 551–556.

Skalinska M. 1971. Experimental and embryological studies in

Skogen KA, Senack L, Holsinger KE.. 2010. Dormancy, small seed size and low germination rates contribute to low recruitment in

Srivastava R, Srivastava GK.. 2002. Autopolyploids of

Stebbins G. 1940. The significance of polyploidy in plant evolution. American Naturalist 74: 54–66.

Tate JA, Symonds VV, Doust AN, et al. 2009. Synthetic polyploids of PubMed

Trávníček P, Eliášova A, Suda J.. 2010. The distribution of cytotypes of Vicia cracca in Central Europe: the changes that have occurred over the last four decades. Preslia 82: 149–163.

Van Dijk P, Van Delden W.. 1990. Evidence for autotetraploidy in

Vera ML. 1997. Effects of altitude and seed size on germination and seedling survival of heathland plants in North Spain. Plant Ecology 133: 101–106.

Wang ZN, You RL, Chen-Zhu XZ.. 2001. Effects of colchicine on the accumulation of vacuolar H

Warner DA, Edwards GE.. 1993. Effects of polyploidy on photosynthesis. Photosynthesis Research 35: 135–147. PubMed

Wu JH, Datson PM, Manako KI, Murray BG.. 2014. Meiotic chromosome pairing behaviour of natural tetraploids and induced autotetraploids of PubMed

Yudin BF. 1970. Capacity for parthenogenesis and effectiveness of selection on the basis of this character in diploid and autotetraploid maize. Genetika 6: 13–22.

Zhang XS, Deng MJ, Fan GQ.. 2014. Differential transcriptome analysis between PubMed PMC

The novel expression of clonality following whole-genome multiplication compensates for reduced fertility in natural autopolyploids

Effect of Whole-Genome Duplication on the Evolutionary Rescue of Sterile Hybrid Monkeyflowers

Effects of polyploidization on the contents of photosynthetic pigments are largely population-specific

Physiological and fitness differences between cytotypes vary with stress in a grassland perennial herb