Quenching of chlorophyll triplet states by carotenoids in algal light-harvesting complexes related to fucoxanthin-chlorophyll protein
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28669083
DOI
10.1007/s11120-017-0416-5
PII: 10.1007/s11120-017-0416-5
Knihovny.cz E-zdroje
- Klíčová slova
- Algae, Energy transfer, Light harvesting, Photoprotection, Photosynthesis, Transient spectroscopy,
- MeSH
- anaerobióza MeSH
- časové faktory MeSH
- chlorofyl metabolismus MeSH
- fluorescenční spektrometrie MeSH
- fotochemické procesy * MeSH
- Heterokontophyta metabolismus MeSH
- karotenoidy metabolismus MeSH
- kinetika MeSH
- proteiny vázající chlorofyl metabolismus MeSH
- světlosběrné proteinové komplexy metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorofyl MeSH
- karotenoidy MeSH
- proteiny vázající chlorofyl MeSH
- světlosběrné proteinové komplexy MeSH
We have used time-resolved absorption and fluorescence spectroscopy with nanosecond resolution to study triplet energy transfer from chlorophylls to carotenoids in a protective process that prevents the formation of reactive singlet oxygen. The light-harvesting complexes studied were isolated from Chromera velia, belonging to a group Alveolata, and Xanthonema debile and Nannochloropsis oceanica, both from Stramenopiles. All three light-harvesting complexes are related to fucoxanthin-chlorophyll protein, but contain only chlorophyll a and no chlorophyll c. In addition, they differ in the carotenoid content. This composition of the complexes allowed us to study the quenching of chlorophyll a triplet states by different carotenoids in a comparable environment. The triplet states of chlorophylls bound to the light-harvesting complexes were quenched by carotenoids with an efficiency close to 100%. Carotenoid triplet states were observed to rise with a ~5 ns lifetime and were spectrally and kinetically homogeneous. The triplet states were formed predominantly on the red-most chlorophylls and were quenched by carotenoids which were further identified or at least spectrally characterized.
Biological Centre Czech Academy of Sciences 370 05 České Budějovice Czech Republic
Faculty of Science University of South Bohemia 370 05 České Budějovice Czech Republic
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