The soil chromophyte alga Xanthonema (X.) debile contains only non-carbonyl carotenoids and Chl-a. X. debile has an antenna system denoted Xanthophyte light-harvesting complex (XLH) that contains the carotenoids diadinoxanthin, heteroxanthin, and vaucheriaxanthin. The XLH pigment stoichiometry was calculated by chromatographic techniques and the pigment-binding structure studied by resonance Raman spectroscopy. The pigment ratio obtained by HPLC was found to be close to 8:1:2:1 Chl-a:heteroxanthin:diadinoxanthin:vaucheriaxanthin. The resonance Raman spectra suggest the presence of 8-10 Chl-a, all of which are 5-coordinated to the central Mg, with 1-3 Chl-a possessing a macrocycle distorted from the relaxed conformation. The three populations of carotenoids are in the all-trans configuration. Vaucheriaxanthin absorbs around 500-530 nm, diadinoxanthin at 494 nm and heteroxanthin at 487 nm at 4.5 K. The effective conjugation length of heteroxanthin and diadinoxanthin has been determined as 9.4 in both cases; the environment polarizability of the heteroxanthin and diadinoxanthin binding pockets is 0.270 and 0.305, respectively.

Biology Centre Czech Academy of Sciences Branisovska 31 370 05 Ceske Budejovice Czech Republic

Faculty of Science University of South Bohemia Branisovska 1760 370 05 Ceske Budejovice Czech Republic

Institute for Integrative Biology of the Cell CEA CNRS Université Paris Saclay 91198 Gif sur Yvette cedex France

Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 People's Republic of China

Zobrazit více v PubMed

Nature. 2007 Nov 22;450(7169):575-8 PubMed

J Biol Chem. 2013 Jun 28;288(26):18758-65 PubMed

J Biol Chem. 2017 Jan 27;292(4):1396-1403 PubMed

Biochim Biophys Acta. 1996 Dec 18;1277(3):243-52 PubMed

Sci Rep. 2015 May 28;5:10134 PubMed

J Biol Chem. 2000 Jul 21;275(29):22031-6 PubMed

J Am Chem Soc. 1973 Jul 11;95(14):4493-501 PubMed

Science. 1996 Jun 21;272(5269):1788-91 PubMed

J Phys Chem B. 2013 Sep 26;117(38):11015-21 PubMed

Biochim Biophys Acta. 2015 Jan;1847(1):12-8 PubMed

Photosynth Res. 2017 Oct;134(1):51-58 PubMed

Photosynth Res. 2010 Nov;106(1-2):57-71 PubMed

Photosynth Res. 2011 May;108(1):25-32 PubMed

Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):534-43 PubMed

Photosynth Res. 2005 Nov;86(1-2):5-24 PubMed

Plant Physiol. 2007 Apr;143(4):1802-16 PubMed

BMC Evol Biol. 2010 Jul 30;10:233 PubMed

Nature. 2004 Mar 18;428(6980):287-92 PubMed

Biochim Biophys Acta. 2010 May;1797(5):543-9 PubMed

Photosynth Res. 2008 Feb-Mar;95(2-3):229-35 PubMed

J Phys Chem A. 2015 Jan 8;119(1):56-66 PubMed

Biochim Biophys Acta. 2016 Nov;1857(11):1759-1765 PubMed

J Phys Chem A. 2014 Mar 13;118(10):1817-25 PubMed

J Phys Chem B. 2012 Aug 2;116(30):8880-9 PubMed

Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):636-40 PubMed

Spectrochim Acta A Mol Biomol Spectrosc. 2011 Apr;78(4):1261-5 PubMed

Photosynth Res. 2005 Nov;86(1-2):241-50 PubMed

Biochemistry. 2006 Jul 18;45(28):8516-26 PubMed

J R Soc Interface. 2017 Oct;14(135):null PubMed

Biochemistry. 1998 Feb 24;37(8):2450-7 PubMed

J Phys Chem B. 2009 Sep 17;113(37):12565-74 PubMed

Photosynth Res. 2018 Mar;135(1-3):213-225 PubMed

Biochim Biophys Acta. 2011 Aug;1807(8):864-77 PubMed

Biochim Biophys Acta. 1977 Jun 9;460(3):408-30 PubMed

Mol Gen Genet. 1995 Feb 20;246(4):455-64 PubMed

Photosynth Res. 2016 Dec;130(1-3):137-150 PubMed

Integrated overview of stramenopile ecology, taxonomy, and heterotrophic origin

Electronic and Vibrational Properties of Allene Carotenoids

Modeling Dynamic Conformations of Organic Molecules: Alkyne Carotenoids in Solution