Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)
Status PubMed-not-MEDLINE Jazyk angličtina Země Německo Médium electronic-ecollection
Typ dokumentu časopisecké články
PubMed
28702366
PubMed Central
PMC5496743
DOI
10.1016/j.bdq.2017.04.001
PII: S2214-7535(16)30028-6
Knihovny.cz E-zdroje
- Klíčová slova
- Data analysis, GenEx software, Limit of detection, Limit of quantification, LoD, LoQ, MIQE, Quality control, Real-time PCR, Replicates, Standardization, qPCR,
- Publikační typ
- časopisecké články MeSH
Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has yet to win broad acceptance in routine practice. This requires a means to unambiguously assess the performance of specific qPCR analyses. Here we present methods to determine the limit of detection (LoD) and the limit of quantification (LoQ) as applicable to qPCR. These are based on standard statistical methods as recommended by regulatory bodies adapted to qPCR and complemented with a novel approach to estimate the precision of LoD.
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