The development of a butyrylcholinesterase porous pellet for innovative detection of cholinesterase inhibitors
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28917965
DOI
10.1016/j.ejps.2017.09.015
PII: S0928-0987(17)30507-9
Knihovny.cz E-zdroje
- Klíčová slova
- Detection, Immobilization, Neusilin, Pellets, Physostigmine, Sarin,
- MeSH
- butyrylcholinesterasa chemie MeSH
- celulosa chemie MeSH
- cholinesterasové inhibitory chemie MeSH
- enzymy imobilizované chemie MeSH
- fysostigmin chemie MeSH
- lékové formy MeSH
- nosiče léků chemie MeSH
- pomocné látky chemie MeSH
- poréznost MeSH
- povidon chemie MeSH
- sarin MeSH
- silikáty chemie MeSH
- sloučeniny hliníku chemie MeSH
- sloučeniny hořčíku chemie MeSH
- tvrdost MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aluminum magnesium silicate MeSH Prohlížeč
- butyrylcholinesterasa MeSH
- celulosa MeSH
- cholinesterasové inhibitory MeSH
- enzymy imobilizované MeSH
- fysostigmin MeSH
- lékové formy MeSH
- microcrystalline cellulose MeSH Prohlížeč
- nosiče léků MeSH
- pomocné látky MeSH
- povidon MeSH
- sarin MeSH
- silikáty MeSH
- sloučeniny hliníku MeSH
- sloučeniny hořčíku MeSH
The aim of the presented research was the preparation of an innovative carrier with significantly improved properties for the fast and sensitive detection of cholinesterase inhibitors such as nerve agents. This innovative carrier was in the form of spherical pellets containing different amounts of Neusilin. Neusilin is a synthetic and amorphous form of magnesium aluminometasilicate with a high specific surface area, and the immobilized enzyme butyrylcholinesterase with an activity of 50nkat·g-1. Pellets were prepared by the extrusion-spheronization method and dried in a hot air oven under two conditions - at 30°C for 72h and at 60°C for 24h. Dried pellets were consequently impregnated with a solution containing butyrylcholinesterase. Impregnated pellets were evaluated for their quality parameters, enzymatic activity and inhibition. Activity and inhibition were tested according to the standard Ellman's method. It was observed that the addition of Neusilin significantly increased the hardness, intraparticular porosity, sphericity and activity of the carriers as well as intensity of the color transition. Therefore it is shown that these carriers have unquestionable advantages over common carriers of their kind. Drying temperatures have been shown to have no effect on properties of pellets except for a change in their size. Results were confirmed by statistical evaluation using ANOVA and PCA.
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