Technical Review: Cytogenetic Tools for Studying Mitotic Chromosomes
Language English Country United States Media print
Document type Journal Article, Review, Research Support, Non-U.S. Gov't
- Keywords
- Antibodies, Cell synchronization, Chromosome spreads, Cytogenetics, FISH, Immunostaining, Laser microdissection,
- MeSH
- Chromosomes, Plant genetics MeSH
- Cytogenetic Analysis methods MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Immunohistochemistry methods MeSH
- Mitosis MeSH
- Chromatin Assembly and Disassembly MeSH
- Plants genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
Significant advances in chromosome preparation and other techniques have greatly increased the potential of plant cytogenetics in recent years. Increase in longitudinal resolution using DNA extended fibers as well as new developments in imaging and signal amplification technologies have enhanced the ability of FISH to detect small gene targets. The combination of fluorescence in situ hybridization with immunocytochemistry allows the investigation of cell events, chromosomal rearrangements and chromatin features typical for plant nuclei. Chromosome manipulation techniques using microdissection and flow sorting have accelerated the analysis of complex plant genomes. Together, the different cytogenetic approaches are invaluable for the unravelling of detailed structures of plant chromosomes, which are of utmost importance for the study of genome properties, DNA replication and gene regulation. In this technical review, different cytogenetic approaches are discussed for the analysis of plant chromosomes, with a focus on mitotic chromosomes.
References provided by Crossref.org
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