Developmental transitions and stress reactions in both eukaryotes and prokaryotes are tightly linked with fast and localized modifications in concentrations of reactive oxygen and nitrogen species (ROS and RNS). Fluorescent microscopic analyses are widely applied to detect localized production of ROS and RNS in vivo. In this mini-review we discuss the biological characteristics of studied material (cell wall, extracellular matrix, and tissue complexity) and its handling (concentration of probes, effect of pressure, and higher temperature) which influence results of histochemical staining with "classical" fluorochromes. Future perspectives of ROS and RNS imaging with newly designed probes are briefly outlined.

Department of Biochemistry Faculty of Science Palacký University Olomouc Olomouc Czechia

Department of Botany Faculty of Science Palacký University Olomouc Olomouc Czechia

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Reactive Oxygen Species as a Response to Wounding: In Vivo Imaging in Arabidopsis thaliana

Data on detection of singlet oxygen, hydroxyl radical and organic radical in Arabidopsis thaliana

Singlet oxygen imaging using fluorescent probe Singlet Oxygen Sensor Green in photosynthetic organisms