PBP2a in β-Lactam-Resistant Laboratory Mutants and Clinical Isolates: Disruption Versus Reduced Penicillin Affinity
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29195053
DOI
10.1089/mdr.2017.0302
Knihovny.cz E-zdroje
- Klíčová slova
- PBP2a, Streptococcus pneumoniae, mosaic gene, penicillin resistance,
- MeSH
- bakteriální geny genetika MeSH
- bakteriální proteiny genetika MeSH
- beta-laktamy farmakologie MeSH
- DNA bakterií genetika MeSH
- lidé MeSH
- mikrobiální testy citlivosti metody MeSH
- mutace genetika MeSH
- peniciliny farmakologie MeSH
- peptidsynthasy genetika MeSH
- proteiny vázající penicilin genetika MeSH
- rezistence na penicilin genetika MeSH
- Streptococcus pneumoniae účinky léků genetika MeSH
- transportní proteiny genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Jihoafrická republika MeSH
- Maďarsko MeSH
- Názvy látek
- bakteriální proteiny MeSH
- beta-laktamy MeSH
- DNA bakterií MeSH
- peniciliny MeSH
- penicillin-binding protein 2a, Streptococcus MeSH Prohlížeč
- peptidsynthasy MeSH
- proteiny vázající penicilin MeSH
- transportní proteiny MeSH
Alterations in PBP2a have been recognized in cefotaxime-resistant laboratory mutants and β-lactam-resistant clinical isolates of Streptococcus pneumoniae. DNA sequencing revealed fundamental differences between these two settings. Internal stop codons in pbp2a occurred in all three laboratory mutants analyzed, caused by a mutation in pbp2a of mutant C604, and tandem duplications within pbp2a resulting in premature stop codons in another two mutants C403 and C406. In contrast, mosaic PBP2a genes were observed in several penicillin-resistant clinical isolates from South Africa, the Czech Republic, Hungary, and in the clone Poland23F-16, with sequence blocks diverging from sensitive strains by over 4%. Most of these pbp2a variants except pbp2a from the South African strain contained sequences related to pbp2a of Streptococcus mitis B6, confirming that this species serves as reservoir for penicillin-resistance determinants.
BA Impfstoffe GmbH Ibbenbüren Germany
Department of Medical Microbiology German National Reference Center for Streptococci Aachen Germany
Department of Microbiology University of Kaiserslautern Kaiserslautern Germany
School of Engineering University of Applied Sciences Saarbrücken Germany
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