Stress affects expression of the clock gene Bmal1 in the suprachiasmatic nucleus of neonatal rats via glucocorticoid-dependent mechanism
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
29266826
DOI
10.1111/apha.13020
Knihovny.cz E-zdroje
- Klíčová slova
- clock genes, development, glucocorticoids, mifepristone, restricted feeding, stress, suprachiasmatic nuclei,
- MeSH
- antagonisté hormonů farmakologie MeSH
- cirkadiánní hodiny * účinky léků genetika MeSH
- druhová specificita MeSH
- fotoperioda MeSH
- glukokortikoidy krev MeSH
- laktace MeSH
- matka - expozice noxám MeSH
- mifepriston farmakologie MeSH
- novorozená zvířata MeSH
- nucleus suprachiasmaticus účinky léků metabolismus patofyziologie MeSH
- potkani inbrední SHR MeSH
- potkani Wistar MeSH
- psychický stres genetika metabolismus patofyziologie MeSH
- receptory glukokortikoidů antagonisté a inhibitory metabolismus MeSH
- transkripční faktory ARNTL genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- antagonisté hormonů MeSH
- Arntl protein, rat MeSH Prohlížeč
- glukokortikoidy MeSH
- mifepriston MeSH
- receptory glukokortikoidů MeSH
- transkripční faktory ARNTL MeSH
AIM: The reactivity of the circadian clock in the suprachiasmatic nuclei (SCN) to stressful stimuli has been controversial but most studies have confirmed the resilience of the SCN to stress. We tested the hypothesis that during a critical period shortly after birth, the developing SCN clock is affected by glucocorticoids. METHODS: Mothers of 2 rat strains with different sensitivities to stress, that is Wistar rats and spontaneously hypertensive rats (SHR), and their pups were exposed to stressful stimuli every day from delivery, and clock gene expression profiles were detected in the 4-day-old pups' SCN. Levels of glucocorticoids in plasma were measured by LC-MS/MS. The glucocorticoid receptors antagonist mifepristone was administered to pups to block the effect of the glucocorticoids. RESULTS: The glucocorticoid receptors were detected at the mRNA and protein levels in the SCN of 4-day-old pups. The exposure of mothers to stressful stimuli elevated their plasma glucocorticoid levels. In Wistar rat pups, combination of daily maternal stress with their manipulation increased the plasma glucocorticoid levels and shifted the Bmal1 rhythm in the SCN which was completely blocked by mifepristone. In contrast, in SHR pups, maternal stress on its own caused phase shift of the Bmal1 expression rhythm in the SCN but the effect was mediated via glucocorticoid-independent mechanism. The Per1 and Per2 expression profiles remained phase-locked to the light/dark cycle. CONCLUSION: The results demonstrate that the SCN is sensitive to stressful stimuli early after birth in pups maintained under light/dark conditions and the effect is mediated via glucocorticoid-dependent pathways.
Citace poskytuje Crossref.org
GENBANK
AB_002108, NM_031678, AB012600
