Molecular epidemiology of Trichophyton quinckeanum - a zoophilic dermatophyte on the rise
Language English Country Germany Media print
Document type Journal Article
PubMed
29314679
DOI
10.1111/ddg.13408
Knihovny.cz E-resources
- MeSH
- Bacteriological Techniques MeSH
- Dermatomycoses diagnosis epidemiology transmission MeSH
- Child MeSH
- Adult MeSH
- Peptide Elongation Factor 1 genetics MeSH
- Cats microbiology MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Molecular Epidemiology * MeSH
- Cross-Sectional Studies MeSH
- Sequence Analysis, DNA MeSH
- Tinea diagnosis epidemiology transmission MeSH
- Trichophyton classification genetics pathogenicity MeSH
- Zoonoses diagnosis transmission MeSH
- Tooth, Nonvital MeSH
- Animals MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Cats microbiology MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Peptide Elongation Factor 1 MeSH
BACKGROUND: Formerly only referred to as a subspecies (T. mentagrophytes var. quinckeanum), T. quinckeanum once again constitutes a distinct species according to the updated taxonomy of dermatophytes. PATIENTS AND METHODS: During routine diagnostic tests conducted at the Mycology Laboratory, Mölbis, Germany, between 11/2013 to 1/2017 (three years and three months), all specimens sent in were examined for T. quinckeanum. Molecular biology methods employed included: 1) DNA hybridization (PCR ELISA), 2) gene sequencing of the ITS region and TEF-1α, and 3) in some cases, MALDI-TOF mass spectrometry. RESULTS: Overall, 62 strains of T. quinckeanum were found. Sixty-eight percent of patients were female; 43 % were children and adolescents (≤ 19 years of age). Cats were a frequent source of infection. Sequencing of all 62 strains revealed a concordance of 100 % with T. quinckeanum sequences contained in the NCBI database. Isolates analyzed by MALDI-TOF mass spectrometry showed specific spectra. CONCLUSIONS: In Germany, the zoophilic dermatophyte T. quinckeanum currently appears to be more prevalent than expected. T. quinckeanum strains were isolated from children and adults with dermatomycosis and tinea capitis. Sources of infection with T. quinckeanum include small rodents (mice), horses, and - remarkably commonly - cats. Given that unequivocal morphological identification of this dermatophyte is not always possible, molecular methods have to be employed in the diagnosis.
Laboratory for Medical Microbiology Mölbis Germany
Microbiological Laboratory R and D Bioanalytics Bruker Daltonik GmbH Bremen Germany
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