Molecular epidemiology of Trichophyton quinckeanum - a zoophilic dermatophyte on the rise
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články
PubMed
29314679
DOI
10.1111/ddg.13408
Knihovny.cz E-zdroje
- MeSH
- bakteriologické techniky MeSH
- dermatomykózy diagnóza epidemiologie přenos MeSH
- dítě MeSH
- dospělí MeSH
- elongační faktor 1 genetika MeSH
- kočky mikrobiologie MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- molekulární epidemiologie * MeSH
- průřezové studie MeSH
- sekvenční analýza DNA MeSH
- tinea diagnóza epidemiologie přenos MeSH
- Trichophyton klasifikace genetika patogenita MeSH
- zoonózy diagnóza přenos MeSH
- zuby neživé MeSH
- zvířata MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- kočky mikrobiologie MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- elongační faktor 1 MeSH
BACKGROUND: Formerly only referred to as a subspecies (T. mentagrophytes var. quinckeanum), T. quinckeanum once again constitutes a distinct species according to the updated taxonomy of dermatophytes. PATIENTS AND METHODS: During routine diagnostic tests conducted at the Mycology Laboratory, Mölbis, Germany, between 11/2013 to 1/2017 (three years and three months), all specimens sent in were examined for T. quinckeanum. Molecular biology methods employed included: 1) DNA hybridization (PCR ELISA), 2) gene sequencing of the ITS region and TEF-1α, and 3) in some cases, MALDI-TOF mass spectrometry. RESULTS: Overall, 62 strains of T. quinckeanum were found. Sixty-eight percent of patients were female; 43 % were children and adolescents (≤ 19 years of age). Cats were a frequent source of infection. Sequencing of all 62 strains revealed a concordance of 100 % with T. quinckeanum sequences contained in the NCBI database. Isolates analyzed by MALDI-TOF mass spectrometry showed specific spectra. CONCLUSIONS: In Germany, the zoophilic dermatophyte T. quinckeanum currently appears to be more prevalent than expected. T. quinckeanum strains were isolated from children and adults with dermatomycosis and tinea capitis. Sources of infection with T. quinckeanum include small rodents (mice), horses, and - remarkably commonly - cats. Given that unequivocal morphological identification of this dermatophyte is not always possible, molecular methods have to be employed in the diagnosis.
Laboratory for Medical Microbiology Mölbis Germany
Microbiological Laboratory R and D Bioanalytics Bruker Daltonik GmbH Bremen Germany
Citace poskytuje Crossref.org
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