Agrobacterium bohemicum sp. nov. isolated from poppy seed wastes in central Bohemia
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29402492
DOI
10.1016/j.syapm.2018.01.003
PII: S0723-2020(18)30023-7
Knihovny.cz E-zdroje
- Klíčová slova
- ANI, Agrobacterium, Codeine, MLSA, Papaver somniferum, Taxonomy,
- MeSH
- Agrobacterium klasifikace genetika izolace a purifikace MeSH
- bakteriální geny MeSH
- biotransformace MeSH
- DNA bakterií genetika MeSH
- fylogeneze * MeSH
- hybridizace nukleových kyselin MeSH
- mastné kyseliny chemie MeSH
- multilokusová sekvenční typizace MeSH
- Papaver mikrobiologie MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- semena rostlinná mikrobiologie MeSH
- ubichinon analogy a deriváty chemie MeSH
- zastoupení bazí MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- coenzyme Q10 MeSH Prohlížeč
- DNA bakterií MeSH
- mastné kyseliny MeSH
- RNA ribozomální 16S MeSH
- ubichinon MeSH
Two non-pathogenic strains R89-1 and R90T isolated from poppy seed (Papaver somniferum L.) wastes were phenotypically and genotypically characterized. Multilocus sequence analysis (MLSA) was conducted with six genes (atpD, glnA, gyrB, recA, rpoB, 16S rRNA). The strains represented a new species which clustered with Agrobacterium rubi NBRC 13261T and Agrobacterium skierniewicense Ch11T type strains. MLSA was further accompanied by whole-genome phylogeny, in silico DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses for both strains. ANI and dDDH values were deep below the species delineation threshold. Phenotypic features of the novel strains unequivocally allowed their differentiation from all other Agrobacterium species. Unlike other agrobacteria, the strains were salt sensitive and were able to biotransform morphine alkaloids. The dominant cellular fatty acids are 18:1 w7c, 16:0 and 12:0 aldehyde/16:1 iso I/14:0 3OH summed in feature 2 and the major respiratory quinine is Q-10 (87%). The DNA G+C content is 56mol%. Microbial community analysis indicated probable association with P. somniferum plant material. Altogether, these characteristics showed that strains R90T and R89-1 represent a new species of the genus Agrobacterium which we propose to name Agrobacterium bohemicum. The type strain of A. bohemicum is R90T (=CCM 8736T=DSM 104667T).
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