Novel injectable gellan gum hydrogel composites incorporating Zn- and Sr-enriched bioactive glass microparticles: High-resolution X-ray microcomputed tomography, antibacterial and in vitro testing
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29489058
DOI
10.1002/term.2654
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální polysacharidy farmakologie MeSH
- hydrogely farmakologie MeSH
- injekce MeSH
- ionty MeSH
- keramika farmakologie MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus účinky léků MeSH
- mikrobiální testy citlivosti MeSH
- nádorové buněčné linie MeSH
- rentgenová mikrotomografie * MeSH
- sklo MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- stroncium chemie MeSH
- zinek chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- bakteriální polysacharidy MeSH
- bioactive glass 45S5 MeSH Prohlížeč
- gellan gum MeSH Prohlížeč
- hydrogely MeSH
- ionty MeSH
- stroncium MeSH
- zinek MeSH
Mineralization of hydrogel biomaterials is desirable to improve their suitability as materials for bone regeneration. In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles. This resulted in novel injectable, self-gelling composites of GG hydrogels containing 20% bioactive glass. Gelation occurred within 20 min. Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff. X-ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels. The widely used melt-derived 45S5 preparation served as a standard and was compared with a calcium-rich, sol-gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5). A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5. Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast-like cells and were considerably more cytocompatible than 45S5. All composites underwent mineralization with calcium-deficient hydroxyapatite upon incubation in simulated body fluid. The extent of mineralization appeared to be greatest for composites containing A2Zn5 and 45S5. The results underline the importance of the choice of bioactive glass when preparing injectable, self-gelling composites.
Centre for Nano and Biophotonics Ghent University Ghent Belgium
Centre for Organismal Studies University of Heidelberg Heidelberg Germany
Department of Analytical Chemistry Ghent University Ghent Belgium
Department of Molecular Biotechology Ghent University Ghent Belgium
Engineering Department Lancaster University Lancaster UK
Materials Science Institute Lancaster University Lancaster UK
Citace poskytuje Crossref.org
Hybrid Polymer-Inorganic Materials with Hyaluronic Acid as Controlled Antibiotic Release Systems
