UVA-photoprotective potential of silymarin and silybin
Language English Country Germany Media print-electronic
Document type Journal Article
Grant support
GACR 15-10897S
Grantová Agentura České Republiky
RVO 61989592
Univerzita Palackého v Olomouci
IGA_LF_2016_012
Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic
IGA_LF_2017_011
Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic
PubMed
29564550
DOI
10.1007/s00403-018-1828-6
PII: 10.1007/s00403-018-1828-6
Knihovny.cz E-resources
- Keywords
- Dermal fibroblasts, Flavonolignan, Heat shock protein, Metalloproteinase-1, Oxidative damage, SPF,
- MeSH
- Fibroblasts drug effects pathology radiation effects MeSH
- Glutathione metabolism MeSH
- Heme Oxygenase-1 metabolism MeSH
- Caspase 3 metabolism MeSH
- Cells, Cultured MeSH
- Skin pathology radiation effects MeSH
- Humans MeSH
- Matrix Metalloproteinase 1 metabolism MeSH
- DNA Damage MeSH
- Primary Cell Culture MeSH
- HSP70 Heat-Shock Proteins metabolism MeSH
- Radiation Injuries drug therapy MeSH
- Reactive Oxygen Species metabolism MeSH
- Silybin MeSH
- Silymarin therapeutic use MeSH
- Sunlight MeSH
- Ultraviolet Rays adverse effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Glutathione MeSH
- Heme Oxygenase-1 MeSH
- HMOX1 protein, human MeSH Browser
- Caspase 3 MeSH
- Matrix Metalloproteinase 1 MeSH
- HSP70 Heat-Shock Proteins MeSH
- Reactive Oxygen Species MeSH
- Silybin MeSH
- Silymarin MeSH
Exposure to solar radiation is a major cause of environmental human skin damage. The main constituent of solar UV light is UVA radiation (320-400 nm); however, the need for protection against UVA has been marginalized for a long time. As a result, there is still a lack of useful agents for UVA protection. In this study, the effect of silymarin (SM) and its main constituent silybin (SB) pre-treatment on UVA-stimulated damage to primary human dermal fibroblasts were carried out. The cells were pre-treated for 1 h with SB or SM and then were exposed to UVA light, using a solar simulator. The effect of SB and SM on reactive oxygen species (ROS) and glutathione (GSH) level, caspase-3 activity, single-strand breaks (SSB) formation and protein level of matrix metalloproteinase-1 (MMP-1), heme oxygenase-1 (HO-1), and heat shock protein (HSP70) was evaluated. Treatment with both SM and SB resulted in a reduction in UVA-stimulated ROS generation and SSB production, as well as in the prevention of GSH depletion, a decrease in the activation of caspase-3 and protein level of MMP-1. They also moderately increased HO-1 level and reduced HSP70 level. Our data showed that both SM and SB are non-phototoxic and have UVA-photoprotective potential and could be useful agents for UV-protective dermatological preparations.
References provided by Crossref.org
Photoprotective properties of new derivatives of kinetin
Skin Protective Activity of Silymarin and its Flavonolignans