Expression of Regeneration-Associated Proteins in Primary Sensory Neurons and Regenerating Axons After Nerve Injury-An Overview
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
Grant support
16-08508S
The Czech Science Foundation - International
PubMed
29740961
DOI
10.1002/ar.23843
Knihovny.cz E-resources
- Keywords
- degeneration, peripheral nerve, regeneration, therapies,
- MeSH
- Axons metabolism MeSH
- Biomarkers metabolism MeSH
- Stress, Physiological MeSH
- Cornified Envelope Proline-Rich Proteins metabolism MeSH
- Membrane Proteins metabolism MeSH
- Microtubule Proteins MeSH
- Sensory Receptor Cells classification physiology MeSH
- GAP-43 Protein metabolism MeSH
- Nerve Regeneration * MeSH
- Ganglia, Spinal cytology metabolism MeSH
- Carrier Proteins metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Biomarkers MeSH
- Cornified Envelope Proline-Rich Proteins MeSH
- Membrane Proteins MeSH
- Microtubule Proteins MeSH
- GAP-43 Protein MeSH
- Sprr1a protein, mouse MeSH Browser
- Stmn2 protein, rat MeSH Browser
- Carrier Proteins MeSH
Peripheral nerve injury results in profound alterations of the affected neurons resulting from the interplay between intrinsic and extrinsic molecular events. Restarting the neuronal regenerative program is an important prerequisite for functional recovery of the injured peripheral nerve. The primary sensory neurons with their cell bodies in the dorsal root ganglia provide a useful in vivo and in vitro model for studying the mechanisms that regulate intrinsic neuronal regeneration capacity following axotomy. These studies frequently need to indicate the regenerative status of the corresponding neurons. We summarize the critical issues regarding immunohistochemical detection of several regeneration-associated proteins as markers for the initiation of the regeneration program in rat primary sensory neurons and indicators of axon regeneration in the peripheral nerves. This overview also includes our own results of GAP43 and SCG10 expression in different DRG neurons following double immunostaining with molecular markers of neuronal subpopulations (NF200, CGRP, and IB4) as well as transcription factors (ATF3 and activated STAT3) following unilateral sciatic nerve injury. Anat Rec, 301:1618-1627, 2018. © 2018 Wiley Periodicals, Inc.
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