Up-regulation of μ-, δ- and κ-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29957382
DOI
10.1016/j.jneuroim.2018.05.008
PII: S0165-5728(18)30077-8
Knihovny.cz E-resources
- Keywords
- Concanavalin A, G proteins, Opioids receptors, Rat spleen lymphocytes, Up-regulation, β-arrestin-1/2,
- MeSH
- Concanavalin A pharmacology MeSH
- Rats MeSH
- Lymphocytes drug effects metabolism MeSH
- Mitogens pharmacology MeSH
- Rats, Wistar MeSH
- Receptors, Opioid, delta biosynthesis drug effects MeSH
- Receptors, Opioid, kappa biosynthesis drug effects MeSH
- Receptors, Opioid, mu biosynthesis drug effects MeSH
- Spleen cytology drug effects metabolism MeSH
- Up-Regulation MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Concanavalin A MeSH
- Mitogens MeSH
- Receptors, Opioid, delta MeSH
- Receptors, Opioid, kappa MeSH
- Receptors, Opioid, mu MeSH
Regulation of μ-, δ- and κ-opioid receptor protein level in spleen lymphocytes when stimulated by mitogen is not known. To answer the question whether these cells do express opioid receptor (OR) proteins, primary, fresh rat spleen lymphocytes were prepared and stimulated for 48 h with mitogenic dose of Con A. The unstimulated lymphocytes did not express μ- and δ-OR proteins in detectable amounts, however, stimulation with Con A resulted in appearance of clearly detectable immunoblot signals of both μ-OR and δ-OR. κ-OR were detected already in primary cells and increased 2.4-fold in Con A-stimulated cells. These results were supported by data obtained by flow cytometry analysis indicating a dramatic increase in number of μ-, δ- and κ-OR expressing cells after mitogen stimulation. The newly synthesized μ-, δ- and κ-OR in Con A-stimulated spleen lymphocytes were present in the cells interior and not functionally mature, at least in terms of their ability to enhance activity of trimeric G proteins determined by three different protocols of agonist-stimulated, high-affinity [35S]GTPγS binding assay. The up-regulation of μ-, δ- and κ-OR was associated with specific decrease of their cognate trimeric G proteins, Gi1α/Gi2α; the other Gα and Gβ subunits were unchanged. The level of β-arrestin-1/2 was also decreased in Con A-stimulated splenocytes. We conclude that up-regulation of OR expression level in spleen lymphocytes by Con A proceeds in conjunction with down-regulation of their intracellular signaling partners, Gi1α/Gi2α proteins and β-arrestin-1/2. These regulatory proteins are expressed in high amounts already in unstimulated cells and decreased by mitogen stimulation.
References provided by Crossref.org
Expression of Opioid Receptors in Cells of the Immune System
