-
Something wrong with this record ?
The synergistic interaction of thermal stress coupled with overstocking strongly modulates the transcriptomic activity and immune capacity of rainbow trout (Oncorhynchus mykiss)
A. Rebl, T. Korytář, A. Borchel, R. Bochert, JE. Strzelczyk, T. Goldammer, M. Verleih,
Language English Country Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Directory of Open Access Journals
from 2011
Free Medical Journals
from 2011
Nature Open Access
from 2011-12-01
PubMed Central
from 2011
Europe PubMed Central
from 2011
ProQuest Central
from 2011-01-01
Open Access Digital Library
from 2011-01-01
Open Access Digital Library
from 2011-01-01
Health & Medicine (ProQuest)
from 2011-01-01
ROAD: Directory of Open Access Scholarly Resources
from 2011
Springer Nature OA/Free Journals
from 2011-12-01
- MeSH
- Glucose metabolism MeSH
- Hemoglobins analysis MeSH
- Hydrocortisone blood MeSH
- Immune System immunology MeSH
- Crowding * MeSH
- Oncorhynchus mykiss genetics immunology MeSH
- Heat-Shock Response * MeSH
- Fish Proteins genetics metabolism MeSH
- Spleen immunology metabolism MeSH
- Gene Expression Profiling MeSH
- Transcriptome * MeSH
- Computational Biology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The objective of the present study is to identify and evaluate informative indicators for the welfare of rainbow trout exposed to (A) a water temperature of 27 °C and (B) a stocking density of 100 kg/m3 combined with a temperature of 27 °C. The spleen-somatic and condition index, haematocrit and the concentrations of haemoglobin, plasma cortisol and glucose revealed non-significant differences between the two stress groups and the reference group 8 days after the onset of the experiments. The transcript abundance of almost 1,500 genes was modulated at least twofold in in the spleen of rainbow trout exposed to a critical temperature alone or a critical temperature combined with crowding as compared to the reference fish. The number of differentially expressed genes was four times higher in trout that were simultaneously challenged with high temperature and crowding, compared to trout challenged with high temperature alone. Based on these sets of differentially expressed genes, we identified unique and common tissue- and stress type-specific pathways. Furthermore, our subsequent immunologic analyses revealed reduced bactericidal and inflammatory activity and a significantly altered blood-cell composition in challenged versus non-challenged rainbow trout. Altogether, our data demonstrate that heat and overstocking exert synergistic effects on the rainbow trout's physiology, especially on the immune system.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20027841
- 003
- CZ-PrNML
- 005
- 20210114152455.0
- 007
- ta
- 008
- 210105s2020 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1038/s41598-020-71852-8 $2 doi
- 035 __
- $a (PubMed)32913268
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Rebl, Alexander $u Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
- 245 14
- $a The synergistic interaction of thermal stress coupled with overstocking strongly modulates the transcriptomic activity and immune capacity of rainbow trout (Oncorhynchus mykiss) / $c A. Rebl, T. Korytář, A. Borchel, R. Bochert, JE. Strzelczyk, T. Goldammer, M. Verleih,
- 520 9_
- $a The objective of the present study is to identify and evaluate informative indicators for the welfare of rainbow trout exposed to (A) a water temperature of 27 °C and (B) a stocking density of 100 kg/m3 combined with a temperature of 27 °C. The spleen-somatic and condition index, haematocrit and the concentrations of haemoglobin, plasma cortisol and glucose revealed non-significant differences between the two stress groups and the reference group 8 days after the onset of the experiments. The transcript abundance of almost 1,500 genes was modulated at least twofold in in the spleen of rainbow trout exposed to a critical temperature alone or a critical temperature combined with crowding as compared to the reference fish. The number of differentially expressed genes was four times higher in trout that were simultaneously challenged with high temperature and crowding, compared to trout challenged with high temperature alone. Based on these sets of differentially expressed genes, we identified unique and common tissue- and stress type-specific pathways. Furthermore, our subsequent immunologic analyses revealed reduced bactericidal and inflammatory activity and a significantly altered blood-cell composition in challenged versus non-challenged rainbow trout. Altogether, our data demonstrate that heat and overstocking exert synergistic effects on the rainbow trout's physiology, especially on the immune system.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a výpočetní biologie $7 D019295
- 650 12
- $a nahuštění v prostoru $7 D003441
- 650 _2
- $a rybí proteiny $x genetika $x metabolismus $7 D029941
- 650 _2
- $a stanovení celkové genové exprese $7 D020869
- 650 _2
- $a glukosa $x metabolismus $7 D005947
- 650 12
- $a reakce na tepelný šok $7 D018869
- 650 _2
- $a hemoglobiny $x analýza $7 D006454
- 650 _2
- $a hydrokortison $x krev $7 D006854
- 650 _2
- $a imunitní systém $x imunologie $7 D007107
- 650 _2
- $a Oncorhynchus mykiss $x genetika $x imunologie $7 D017686
- 650 _2
- $a slezina $x imunologie $x metabolismus $7 D013154
- 650 12
- $a transkriptom $7 D059467
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Korytář, Tomáš $u Institute of Aquaculture and Protection of Waters, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Husova tř. 458/102, 370 05, České Budějovice, Czech Republic.
- 700 1_
- $a Borchel, Andreas $u Sea Lice Research Centre (SLRC), Department of Biology, University of Bergen, Thormøhlensgate 55, 5008, Bergen, Norway.
- 700 1_
- $a Bochert, Ralf $u Institute of Fisheries, State Research Centre of Agriculture and Fisheries, Mecklenburg-Vorpommern (LFA-MV), Südstraße 8, 18375, Born, Germany.
- 700 1_
- $a Strzelczyk, Joanna Ewa $u Institute of Immunology, Federal Research Institute for Animal Health, Friedrich-Loeffler-Institute, 17493, Greifswald-Insel Riems, Germany.
- 700 1_
- $a Goldammer, Tom $u Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany. Faculty of Agriculture and Environmental Sciences, University of Rostock, 18059, Rostock, Germany.
- 700 1_
- $a Verleih, Marieke $u Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany. verleih@fbn-dummerstorf.de.
- 773 0_
- $w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 10, č. 1 (2020), s. 14913
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/32913268 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20210105 $b ABA008
- 991 __
- $a 20210114152453 $b ABA008
- 999 __
- $a ok $b bmc $g 1608176 $s 1119021
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 10 $c 1 $d 14913 $e 20200910 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
- LZP __
- $a Pubmed-20210105
