Up-regulation of μ-, δ- and κ-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29957382
DOI
10.1016/j.jneuroim.2018.05.008
PII: S0165-5728(18)30077-8
Knihovny.cz E-zdroje
- Klíčová slova
- Concanavalin A, G proteins, Opioids receptors, Rat spleen lymphocytes, Up-regulation, β-arrestin-1/2,
- MeSH
- konkanavalin A farmakologie MeSH
- krysa rodu Rattus MeSH
- lymfocyty účinky léků metabolismus MeSH
- mitogeny farmakologie MeSH
- potkani Wistar MeSH
- receptory opiátové delta biosyntéza účinky léků MeSH
- receptory opiátové kappa biosyntéza účinky léků MeSH
- receptory opiátové mu biosyntéza účinky léků MeSH
- slezina cytologie účinky léků metabolismus MeSH
- upregulace MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- konkanavalin A MeSH
- mitogeny MeSH
- receptory opiátové delta MeSH
- receptory opiátové kappa MeSH
- receptory opiátové mu MeSH
Regulation of μ-, δ- and κ-opioid receptor protein level in spleen lymphocytes when stimulated by mitogen is not known. To answer the question whether these cells do express opioid receptor (OR) proteins, primary, fresh rat spleen lymphocytes were prepared and stimulated for 48 h with mitogenic dose of Con A. The unstimulated lymphocytes did not express μ- and δ-OR proteins in detectable amounts, however, stimulation with Con A resulted in appearance of clearly detectable immunoblot signals of both μ-OR and δ-OR. κ-OR were detected already in primary cells and increased 2.4-fold in Con A-stimulated cells. These results were supported by data obtained by flow cytometry analysis indicating a dramatic increase in number of μ-, δ- and κ-OR expressing cells after mitogen stimulation. The newly synthesized μ-, δ- and κ-OR in Con A-stimulated spleen lymphocytes were present in the cells interior and not functionally mature, at least in terms of their ability to enhance activity of trimeric G proteins determined by three different protocols of agonist-stimulated, high-affinity [35S]GTPγS binding assay. The up-regulation of μ-, δ- and κ-OR was associated with specific decrease of their cognate trimeric G proteins, Gi1α/Gi2α; the other Gα and Gβ subunits were unchanged. The level of β-arrestin-1/2 was also decreased in Con A-stimulated splenocytes. We conclude that up-regulation of OR expression level in spleen lymphocytes by Con A proceeds in conjunction with down-regulation of their intracellular signaling partners, Gi1α/Gi2α proteins and β-arrestin-1/2. These regulatory proteins are expressed in high amounts already in unstimulated cells and decreased by mitogen stimulation.
Citace poskytuje Crossref.org
Expression of Opioid Receptors in Cells of the Immune System
