Triple-Color Live Imaging of Mouse Oocytes
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Chromosome, Live imaging, Meiosis, Oocyte, Spindle,
- MeSH
- Spindle Apparatus metabolism MeSH
- Red Fluorescent Protein MeSH
- Microscopy, Fluorescence methods MeSH
- Cells, Cultured MeSH
- Luminescent Proteins metabolism MeSH
- Meiosis * MeSH
- Microtubules metabolism MeSH
- Mice MeSH
- Oocytes cytology physiology MeSH
- Image Processing, Computer-Assisted MeSH
- Chromosome Segregation MeSH
- Tubulin metabolism MeSH
- Green Fluorescent Proteins metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Luminescent Proteins MeSH
- Tubulin MeSH
- Green Fluorescent Proteins MeSH
Meiotic division is a dynamic process that exhibits active interactive behaviors amongst different intracellular structures and components for spindle assembly and chromosome segregation. Understanding the mechanisms of meiotic spindle assembly and chromosome segregation therefore requires a quantitative analysis of spatiotemporal relationships among different structures and components. In this chapter, we describe a method for triple-color live imaging of meiotic division in mouse oocytes. This approach combines the microinjection of RNAs encoding proteins tagged with green and red fluorescent proteins and the visualization of microtubules with the fluorogenic far-red probe SiR-Tubulin. This method enables the simultaneous spatiotemporal mapping of three different components of the spindle and chromosomes, which opens the way to quantitative analysis of their interactive behaviors.
Institute of Animal Physiology and Genetics AS CR Libechov Czech Republic
Laboratory for Chromosome Segregation RIKEN Center for Biosystems Dynamics Research Kobe Japan
References provided by Crossref.org
RanGTP and importin β regulate meiosis I spindle assembly and function in mouse oocytes
