Analysis of the bacterial community from high alkaline (pH > 13) drainage water at a brown mud disposal site near Žiar nad Hronom (Banská Bystrica region, Slovakia) using 454 pyrosequencing
Language English Country United States Media print-electronic
Document type Journal Article
Grant support
no. 26220120001
Research & Development Operational Programme funded by the European Regional Development Fund
VEGA 1/0229/17
Ministry of Education, Science, Research and Sport of Slovak Republic
VVGS-2016-275
Pavol Jozef Safarik University in Kosice, Slovakia
PubMed
30084086
DOI
10.1007/s12223-018-0634-z
PII: 10.1007/s12223-018-0634-z
Knihovny.cz E-resources
- Keywords
- 454 pyrosequencing, Bacteria, Brown mud, Drainage water, Heavy metals, High alkalinity,
- MeSH
- Bacteria classification genetics isolation & purification MeSH
- Biodegradation, Environmental MeSH
- Biodiversity * MeSH
- Water Pollutants, Chemical analysis metabolism MeSH
- Phylogeny MeSH
- Bacterial Physiological Phenomena * MeSH
- Metallurgy MeSH
- Hydrogen-Ion Concentration MeSH
- Microbial Consortia genetics MeSH
- Wastewater chemistry microbiology MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Metals, Heavy analysis metabolism MeSH
- High-Throughput Nucleotide Sequencing * MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Slovakia MeSH
- Names of Substances
- Water Pollutants, Chemical MeSH
- Waste Water MeSH
- RNA, Ribosomal, 16S MeSH
- Metals, Heavy MeSH
Brown mud, as a waste product of the industrial process of aluminum production, represents a great environmental burden due to its toxicity to living organisms. However, some microorganisms are able to survive in this habitat, and they can be used in bioremediation processes. Traditional cultivation methods have a limited capacity to characterize bacterial composition in environmental samples. Recently, next-generation sequencing methods have provided new perspectives on microbial community studies. The aim of this study was to analyze the bacterial community in the drainage water of brown mud disposal site near Žiar nad Hronom (Banská Bystrica region, Slovakia) using 454 pyrosequencing. We obtained 9964 sequences assigned to 163 operational taxonomic units belonging to 10 bacterial phyla. The phylum Proteobacteria showed the highest abundance (80.39%) within the bacterial community, followed by Firmicutes (13.05%) and Bacteroidetes (5.64%). Other bacterial phyla showed an abundance lower than 1%. The classification yielded 85 genera. Sulfurospirillum spp. (45.19%) dominated the bacterial population, followed by Pseudomonas spp. (13.76%) and Exiguobacterium spp. (13.02%). These results indicate that high heavy metals content, high pH, and lack of essential nutrients are the drivers of a dramatic reduction of diversity in the bacterial population in this environment.
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