Bordetella pertussis, the causative agent of whooping cough, has the capability to survive inside the host cells. This process requires efficient adaptation of the pathogen to the intracellular environment and the associated stress. Among the proteins produced by the intracellular B. pertussis we identified a protein (BP0414) that shares homology with MgtC, a protein which was previously shown to be involved in the intracellular survival of other pathogens. To explore if BP0414 plays a role in B. pertussis intracellular survival a mutant strain defective in the production of this protein was constructed. Using standard in vitro growth conditions we found that BP0414 is required for B. pertussis growth under low magnesium availability or low pH, two environmental conditions that this pathogen might face within the host cell. Intracellular survival studies showed that MgtC is indeed involved in B. pertussis viability inside the macrophages. The use of bafilomycin A1, which inhibits phagosome acidification, abolished the survival defect of the mgtC deficient mutant strain suggesting that in intracellular B. pertussis the role of MgtC protein is mainly related to the bacterial adaptation to the acidic conditions found inside the of phagosomes. Overall, this work provides an insight into the importance of MgtC in B. pertussis pathogenesis and its contribution to bacterial survival within immune cells.

CINDEFI Facultad de Ciencias Exactas Universidad Nacional de La Plata La Plata Argentina

Interfaculty Institute for Genetics and Functional Genomics Department of Functional Genomics University Medicine Greifswald Greifswald Germany

Laboratory of Molecular Biology of Bacterial Pathogens Institute of Microbiology of the ASCR v v i Prague Czech Republic

Laboratory of post transcriptional control of gene expression Institute of Microbiology of the ASCR v v i Prague Czech Republic

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Avirulent phenotype promotes Bordetella pertussis adaptation to the intramacrophage environment