Serum C-peptide level correlates with the course of muscle tissue healing in the rabbit model of critical limb ischemia
Jazyk angličtina Země Česko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30198519
DOI
10.5507/bp.2018.048
Knihovny.cz E-zdroje
- Klíčová slova
- C-peptide, critical limb ischemia, cytokines, mesenchymal stem cells, miR-126, tissue healing,
- MeSH
- C-peptid krev MeSH
- cytokiny krev MeSH
- diabetická noha MeSH
- dospělí MeSH
- experimentální diabetes mellitus krev MeSH
- fibróza MeSH
- fyziologická neovaskularizace účinky léků MeSH
- granulační tkáň patologie MeSH
- hojení ran účinky léků fyziologie MeSH
- hybridizace in situ MeSH
- inzulin krev MeSH
- ischemie krev MeSH
- jizva patologie MeSH
- kosterní svaly krevní zásobení účinky léků patologie MeSH
- králíci MeSH
- kultivační média speciální farmakologie MeSH
- lidé MeSH
- mezenchymální kmenové buňky * MeSH
- mikro RNA metabolismus MeSH
- modely nemocí na zvířatech MeSH
- nekróza MeSH
- zadní končetina MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- králíci MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- C-peptid MeSH
- cytokiny MeSH
- inzulin MeSH
- kultivační média speciální MeSH
- mikro RNA MeSH
AIM: The therapeutic potential of adipose-derived stem cell conditioned medium (ASC-CM) was studied in the rabbit model of critical limb ischemia (CLI). METHODS: Rabbits received treatment with ASC-CM or placebo. Gastrocnemius muscle tissue was collected 35 days after ischemia induction. Ischemic changes were evaluated in hematoxylin-eosin stained tissues for early (necrotic lesions/granulation tissue) and late (fibrous scars) phases of tissue repair. The expression of proangiogenic miR-126 was also evaluated using in situ hybridization. The levels of cytokines, insulin, and C-peptide were measured in blood. RESULTS: Early repair phases were observed more often in placebo-treated samples (45.5%) than in ASC-CM-treated ones (22.2%). However, the difference was not statistically significant. We demonstrated a statistically significant positive correlation between the early healing phases in tissue samples and C-peptide levels in peripheral blood. The expression of proangiogenic miR-126 was also shown in a number of structures in all phases of ischemic tissue healing. CONCLUSION: Based on our results, we believe that treatment with ASC-CM has the potential to accelerate the healing process in ischemic tissues in the rabbit model of CLI. The whole healing process was accompanied by miR-126 tissue expression. C-peptide could be used to monitor the course of the tissue healing process.
Center for Cardiovascular Research and Development American Heart of Poland Inc Kostkowice Poland
Department of Deputy Director of Science and Research University Hospital Ostrava Czech Republic
Department of Surgery University Hospital Ostrava Czech Republic
Laboratore AGEL a s Novy Jicin Czech Republic
MEDi CBTD Ostrava Czech Republic
Citace poskytuje Crossref.org
