Serum C-peptide level correlates with the course of muscle tissue healing in the rabbit model of critical limb ischemia
Language English Country Czech Republic Media print-electronic
Document type Journal Article
PubMed
30198519
DOI
10.5507/bp.2018.048
Knihovny.cz E-resources
- Keywords
- C-peptide, critical limb ischemia, cytokines, mesenchymal stem cells, miR-126, tissue healing,
- MeSH
- C-Peptide blood MeSH
- Cytokines blood MeSH
- Diabetic Foot MeSH
- Adult MeSH
- Diabetes Mellitus, Experimental blood MeSH
- Fibrosis MeSH
- Neovascularization, Physiologic drug effects MeSH
- Granulation Tissue pathology MeSH
- Wound Healing drug effects physiology MeSH
- In Situ Hybridization MeSH
- Insulin blood MeSH
- Ischemia blood MeSH
- Cicatrix pathology MeSH
- Muscle, Skeletal blood supply drug effects pathology MeSH
- Rabbits MeSH
- Culture Media, Conditioned pharmacology MeSH
- Humans MeSH
- Mesenchymal Stem Cells * MeSH
- MicroRNAs metabolism MeSH
- Disease Models, Animal MeSH
- Necrosis MeSH
- Hindlimb MeSH
- Animals MeSH
- Check Tag
- Adult MeSH
- Rabbits MeSH
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- C-Peptide MeSH
- Cytokines MeSH
- Insulin MeSH
- Culture Media, Conditioned MeSH
- MicroRNAs MeSH
AIM: The therapeutic potential of adipose-derived stem cell conditioned medium (ASC-CM) was studied in the rabbit model of critical limb ischemia (CLI). METHODS: Rabbits received treatment with ASC-CM or placebo. Gastrocnemius muscle tissue was collected 35 days after ischemia induction. Ischemic changes were evaluated in hematoxylin-eosin stained tissues for early (necrotic lesions/granulation tissue) and late (fibrous scars) phases of tissue repair. The expression of proangiogenic miR-126 was also evaluated using in situ hybridization. The levels of cytokines, insulin, and C-peptide were measured in blood. RESULTS: Early repair phases were observed more often in placebo-treated samples (45.5%) than in ASC-CM-treated ones (22.2%). However, the difference was not statistically significant. We demonstrated a statistically significant positive correlation between the early healing phases in tissue samples and C-peptide levels in peripheral blood. The expression of proangiogenic miR-126 was also shown in a number of structures in all phases of ischemic tissue healing. CONCLUSION: Based on our results, we believe that treatment with ASC-CM has the potential to accelerate the healing process in ischemic tissues in the rabbit model of CLI. The whole healing process was accompanied by miR-126 tissue expression. C-peptide could be used to monitor the course of the tissue healing process.
Center for Cardiovascular Research and Development American Heart of Poland Inc Kostkowice Poland
Department of Deputy Director of Science and Research University Hospital Ostrava Czech Republic
Department of Surgery University Hospital Ostrava Czech Republic
Laboratore AGEL a s Novy Jicin Czech Republic
MEDi CBTD Ostrava Czech Republic
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