HNK-1 in Morphological Study of Development of the Cardiac Conduction System in Selected Groups of Sauropsida
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
KJB6111301
Grant Agency of the Academy of Sciences of the Czech Republic - International
16-02972S and 13-12412S
Grantová Agentura České Republiky - International
PROGRES-Q38 and COST LTC17023, MEYS (LM2015062 Cze
Ministry of Education, Youth and Sports of the Czech Republic - International
13/2003-Czech Republic
NATO Fellowship Programme - International
Czech Academy of Sciences - International
Ministry of Education, Youth and Sports - International
Grant Agency of the Czech Republic - International
PubMed
30306736
DOI
10.1002/ar.23925
Knihovny.cz E-resources
- Keywords
- CD 57, Leu 7, heart, immunohistochemistry, reptile,
- MeSH
- CD57 Antigens immunology metabolism MeSH
- Biomarkers metabolism MeSH
- Coturnix embryology growth & development metabolism MeSH
- Immunohistochemistry MeSH
- Antibodies, Monoclonal immunology MeSH
- Myocardium cytology metabolism MeSH
- Image Processing, Computer-Assisted methods MeSH
- Heart Conduction System anatomy & histology physiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CD57 Antigens MeSH
- Biomarkers MeSH
- Antibodies, Monoclonal MeSH
Human natural killer (HNK)-1 antibody is an established marker of developing cardiac conduction system (CCS) in birds and mammals. In our search for the evolutionary origin of the CCS, we tested this antibody in a variety of sauropsid species (Crocodylus niloticus, Varanus indicus, Pogona vitticeps, Pantherophis guttatus, Eublepharis macularius, Gallus gallus, and Coturnix japonica). Hearts of different species were collected at various stages of embryonic development and studied to map immunoreactivity in cardiac tissues. We performed detection on alternating serial paraffin sections using immunohistochemistry for smooth muscle actin or sarcomeric actin as myocardial markers, and HNK-1 to visualize overall staining pattern and then positivity in specific myocyte populations. We observed HNK-1 expression of various intensity distributed in the extracellular matrix and mesenchymal cell surface of cardiac cushions in most of the examined hearts. Strong staining was found in the cardiac nerve fibers and ganglia in all species. The myocardium of the sinus venosus and the atrioventricular canal exhibited transitory patterns of expression. In the Pogona and Crocodylus hearts, as well as in the Gallus and Coturnix ones, additional expression was detected in a subset of myocytes of the (inter)ventricular septum. These results support the use of HNK-1 as a conserved marker of the CCS and suggest that there is a rudimentary CCS present in developing reptilian hearts. Anat Rec, 302:69-82, 2019. © 2018 Wiley Periodicals, Inc.
Institute of Anatomy Charles University Prague Czech Republic
Institute of Physiology The Czech Academy of Sciences Prague Czech Republic
References provided by Crossref.org
Low incidence of atrial septal defects in nonmammalian vertebrates
