The role of αβ T-cells in spontaneous regression of melanoma tumors in swine
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
30308209
DOI
10.1016/j.dci.2018.10.001
PII: S0145-305X(18)30388-4
Knihovny.cz E-zdroje
- Klíčová slova
- Cell surface molecules, Lymphocyte subpopulations, Melanoma, Porcine immune system, Tumor regression, αβ T-cells,
- MeSH
- antigeny CD4 metabolismus MeSH
- antigeny CD8 metabolismus MeSH
- cytotoxicita imunologická MeSH
- experimentální nádory imunologie MeSH
- imunofenotypizace MeSH
- kultivované buňky MeSH
- lidé MeSH
- melanom imunologie MeSH
- modely nemocí na zvířatech MeSH
- prasata imunologie MeSH
- receptory antigenů T-buněk alfa-beta metabolismus MeSH
- spontánní regrese nádoru MeSH
- T-lymfocyty imunologie MeSH
- tumor infiltrující lymfocyty imunologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny CD4 MeSH
- antigeny CD8 MeSH
- receptory antigenů T-buněk alfa-beta MeSH
Using a porcine model, we describe Melanoma-Associated CD4+CD8hi T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. These MATL possess the CD4+CD8hi phenotype and they have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL) isolated from melanoma loci. Both MATL and CD4+CD8hi TIL have a similar expression of selected markers indicating that they represent effector/memory αβ T-cell subset. Moreover, although TIL also contain CD4-CD8+ T-cells, only CD4+CD8hi TIL expand during melanoma regression. Importantly, TIL isolated from different pigs and different melanoma loci among the same pig have similar composition of CD4/CD8 subsets, indicating that the composition of the MATL and TIL compartment is identical. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression.
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