The role of αβ T-cells in spontaneous regression of melanoma tumors in swine
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30308209
DOI
10.1016/j.dci.2018.10.001
PII: S0145-305X(18)30388-4
Knihovny.cz E-resources
- Keywords
- Cell surface molecules, Lymphocyte subpopulations, Melanoma, Porcine immune system, Tumor regression, αβ T-cells,
- MeSH
- CD4 Antigens metabolism MeSH
- CD8 Antigens metabolism MeSH
- Cytotoxicity, Immunologic MeSH
- Neoplasms, Experimental immunology MeSH
- Immunophenotyping MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Melanoma immunology MeSH
- Disease Models, Animal MeSH
- Swine immunology MeSH
- Receptors, Antigen, T-Cell, alpha-beta metabolism MeSH
- Neoplasm Regression, Spontaneous MeSH
- T-Lymphocytes immunology MeSH
- Lymphocytes, Tumor-Infiltrating immunology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CD4 Antigens MeSH
- CD8 Antigens MeSH
- Receptors, Antigen, T-Cell, alpha-beta MeSH
Using a porcine model, we describe Melanoma-Associated CD4+CD8hi T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. These MATL possess the CD4+CD8hi phenotype and they have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL) isolated from melanoma loci. Both MATL and CD4+CD8hi TIL have a similar expression of selected markers indicating that they represent effector/memory αβ T-cell subset. Moreover, although TIL also contain CD4-CD8+ T-cells, only CD4+CD8hi TIL expand during melanoma regression. Importantly, TIL isolated from different pigs and different melanoma loci among the same pig have similar composition of CD4/CD8 subsets, indicating that the composition of the MATL and TIL compartment is identical. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression.
References provided by Crossref.org
Melanoma-Bearing Libechov Minipig (MeLiM): The Unique Swine Model of Hereditary Metastatic Melanoma
