Brightly Fluorescent 2'-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- adenin analogy a deriváty chemie MeSH
- barvení a značení MeSH
- cytosin chemie MeSH
- deoxyribonukleosidy chemie MeSH
- DNA analýza MeSH
- fluorescenční barviva chemie MeSH
- lidé MeSH
- metylace MeSH
- nádorové buněčné linie MeSH
- optické zobrazování MeSH
- polyfosfáty chemie MeSH
- průtoková cytometrie MeSH
- puriny chemie MeSH
- sloučeniny boru chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene MeSH Prohlížeč
- 7-deazaadenine MeSH Prohlížeč
- 7-deazapurine MeSH Prohlížeč
- adenin MeSH
- cytosin MeSH
- deoxyribonukleosidy MeSH
- DNA MeSH
- fluorescenční barviva MeSH
- polyfosfáty MeSH
- puriny MeSH
- sloučeniny boru MeSH
- triphosphoric acid MeSH Prohlížeč
Synthesis of cytosine, uracil, and 7-deazaadenine 2'-deoxyribonucleosides and triphosphates (dNTPs) bearing hexamethylated phenyl-bodipy fluorophore attached at position 5 of pyrimidines or at position 7 of 7-deazapurine was developed. All the title labeled nucleosides and dNTPs displayed bright green fluorescence with very high quantum yields. The modified dNmBdpTPs were good substrates to diverse DNA polymerases and were used for in vitro enzymatic synthesis of labeled DNA by primer extension or PCR. In combination with cationic cyclodextrin-peptide-based dNTP transporter, the dNmBdpTPs were successfully used for staining of genomic DNA in living cells for applications in confocal microscopy and in flow cytometry. The best performing cytosine nucleotide dCmBdpTP was used to monitor mitosis in live cells.
Citace poskytuje Crossref.org
