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Chloroplasts preferentially take up ferric-citrate over iron-nicotianamine complexes in Brassica napus

Müller, Brigitta
Author Müller, Brigitta Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117, Budapest, Hungary
Kovács, Krisztina
Author Kovács, Krisztina Laboratory of Nuclear Chemistry, Institute of Chemistry, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/A, 1117, Budapest, Hungary
Pham, Hong-Diep
Author Pham, Hong-Diep Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117, Budapest, Hungary
Kavak, Yusuf
Author Kavak, Yusuf Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117, Budapest, Hungary
Pechoušek, Jiři
Author Pechoušek, Jiři Departments of Experimental Physics and Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacký University, Šlechtitelů 27, 783 71, Olomouc, Czech Republic
Machala, Libor
Author Machala, Libor Departments of Experimental Physics and Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacký University, Šlechtitelů 27, 783 71, Olomouc, Czech Republic
Zbořil, Radek
Author Zbořil, Radek Departments of Experimental Physics and Physical Chemistry, Faculty of Science, Regional Centre of Advanced Technologies and Materials, Palacký University, Šlechtitelů 27, 783 71, Olomouc, Czech Republic
Szenthe, Kálmán
Author Szenthe, Kálmán RT-Europe Nonprofit Research Ltd., Vár tér 2, E Building, Mosonmagyaróvár, 9200, Hungary
Abadía, Javier
Author Abadía, Javier Department of Plant Nutrition, Aula Dei Experimental Station, Spanish Council for Scientific Research (CSIC), P.O. Box 13034, 50080, Saragossa, Spain
Fodor, Ferenc
Author Fodor, Ferenc Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117, Budapest, Hungary
Klencsár, Zoltán
Author Klencsár, Zoltán Centre for Energy Research, Hungarian Academy of Sciences, Konkoly Thege Miklós út 29-33, Budapest, 1121, Hungary
Solti, Ádám
Author Solti, Ádám ORCID Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/C, 1117, Budapest, Hungary. adam.solti@ttk.elte.hu

Planta. 2019 Mar ; 249 (3) : 751-763. [epub] 20181031

ISSN 1432-2048 | 0032-0935
Source

Language English Country Germany Media print-electronic

Document type Journal Article

Persistent link https://www.medvik.cz/link/pmid30382344

Grant support
VEKOP-2.3.3-15-2016-00008 Government of Hungary
PD-112047 National Research, Development and Innovation Office, Hungary
PD-111979 National Research, Development and Innovation Office, Hungary
K-124159 National Research, Development and Innovation Office, Hungary
AGL2016-75226-R Spanish Ministry of Economy and Competitiveness
LO1305 Ministry of Education, Youth and Sports of the Czech Republic
BO/00207/15/4 Magyar Tudományos Akadémia

PubMed 30382344
DOI 10.1007/s00425-018-3037-0
PII: 10.1007/s00425-018-3037-0
Knihovny.cz E-resources

Keywords
Bathophenanthroline disulfonate, Ferric chelate reductase, Gene expression, Mössbauer spectroscopy, Oilseed rape,
MeSH
Brassica napus metabolism MeSH
Chloroplasts metabolism MeSH
Real-Time Polymerase Chain Reaction MeSH
Azetidinecarboxylic Acid analogs & derivatives metabolism MeSH
Spectroscopy, Mossbauer MeSH
Transcriptome MeSH
Ferric Compounds metabolism MeSH
Iron metabolism MeSH
Publication type
Journal Article MeSH
Names of Substances
ferric citrate MeSH Browser
Azetidinecarboxylic Acid MeSH
nicotianamine MeSH Browser
Ferric Compounds MeSH
Iron MeSH

Fe uptake machinery of chloroplasts prefers to utilise Fe(III)-citrate over Fe-nicotianamine complexes. Iron uptake in chloroplasts is a process of prime importance. Although a few members of their iron transport machinery were identified, the substrate preference of the system is still unknown. Intact chloroplasts of oilseed rape (Brassica napus) were purified and subjected to iron uptake studies using natural and artificial iron complexes. Fe-nicotianamine (NA) complexes were characterised by 5 K, 5 T Mössbauer spectrometry. Expression of components of the chloroplast Fe uptake machinery was also studied. Fe(III)-NA contained a minor paramagnetic Fe(II) component (ca. 9%), a paramagnetic Fe(III) component exhibiting dimeric or oligomeric structure (ca. 20%), and a Fe(III) complex, likely being a monomeric structure, which undergoes slow electronic relaxation at 5 K (ca. 61%). Fe(II)-NA contained more than one similar chemical Fe(II) environment with no sign of Fe(III) components. Chloroplasts preferred Fe(III)-citrate compared to Fe(III)-NA and Fe(II)-NA, but also to Fe(III)-EDTA and Fe(III)-o,o'EDDHA, and the Km value was lower for Fe(III)-citrate than for the Fe-NA complexes. Only the uptake of Fe(III)-citrate was light-dependent. Regarding the components of the chloroplast Fe uptake system, only genes of the reduction-based Fe uptake system showed high expression. Chloroplasts more effectively utilize Fe(III)-citrate, but hardly Fe-NA complexes in Fe uptake.

Centre for Energy Research Hungarian Academy of Sciences Konkoly Thege Miklós út 29 33 Budapest 1121 Hungary

Department of Plant Nutrition Aula Dei Experimental Station Spanish Council for Scientific Research P O Box 13034 50080 Saragossa Spain

Department of Plant Physiology and Molecular Plant Biology Institute of Biology ELTE Eötvös Loránd University Pázmány Péter sétány 1 C 1117 Budapest Hungary

Departments of Experimental Physics and Physical Chemistry Faculty of Science Regional Centre of Advanced Technologies and Materials Palacký University Šlechtitelů 27 783 71 Olomouc Czech Republic

Laboratory of Nuclear Chemistry Institute of Chemistry ELTE Eötvös Loránd University Pázmány Péter sétány 1 A 1117 Budapest Hungary

RT Europe Nonprofit Research Ltd Vár tér 2 E Building Mosonmagyaróvár 9200 Hungary

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